%0 Journal Article
%T High-Speed Coherent Raman Fingerprint Imaging of Biological Tissues
%A Charles H. Camp Jr.
%A Young Jong Lee
%A John M. Heddleston
%A Christopher M. Hartshorn
%A Angela R. Hight Walker
%A Jeremy N. Rich
%A Justin D. Lathia
%A Marcus T. Cicerone
%J Physics
%D 2014
%I arXiv
%R 10.1038/nphoton.2014.145
%X We have developed a coherent Raman imaging platform using broadband coherent anti-Stokes Raman scattering (BCARS) that provides an unprecedented combination of speed, sensitivity, and spectral breadth. The system utilizes a unique configuration of laser sources that probes the Raman spectrum over 3,000 cm$^{-1}$ and generates an especially strong response in the typically weak Raman "fingerprint" region through heterodyne amplification of the anti-Stokes photons with a large nonresonant background (NRB) while maintaining high spectral resolution of $<$ 13 cm$^{-1}$. For histology and pathology, this system shows promise in highlighting major tissue components in a non-destructive, label-free manner. We demonstrate high-speed chemical imaging in two- and three-dimensional views of healthy murine liver and pancreas tissues and interfaces between xenograft brain tumors and the surrounding healthy brain matter.
%U http://arxiv.org/abs/1402.3211v2