%0 Journal Article
%T Comparison of an In-House Quantitative Real-Time PCR and COBAS AmpliPrep/TaqMan Roche for Determination of Viral Load for HIV Type 1 Non-B
%A Erick Ntambwe Kamangu
%A Adawaye Chatte
%A Raphael Boreux
%A Fabrice Susin
%A Richard Lunganza Kalala
%A Georges Lelo Mvumbi
%A Patrick De Mol
%A Marie-Pierre Hayette
%A Dolores Vaira
%J Open Access Library Journal
%V 2
%N 3
%P 1-7
%@ 2333-9721
%D 2015
%I Open Access Library
%R 10.4236/oalib.1101402
%X Context: The in-house techniques or experimental methods are increasingly
recommended for their low-cost reagents for the determination of the Viral Load
(VL) in resource-limited settings. The objective of this study was to compare
the determination of VL from HIV-1 non-B samples by an in-house technique with
the COBAS AmpliPrep/TaqMan version 2.0. Method: In this cross-sectional
study, 39 plasma samples from patients infected with HIV type 1 non-B from N¡¯Djamena
and Kinshasa were used to determine the VL using the two techniques. Results: The
mean values of VL are respectively 4.68 ¡À
1.26 and 4.58 ¡À 1.33 log<sub>10</sub> RNA copies/ml for the COBAS AmpliPrep/TaqMan
assays and the in-house assays. A good correlation (Spearman Correlation) was
obtained, with a coefficient (R<sup>2</sup>) of 0.9452. Conclusion: This study
demonstrates that there is no significant difference between the results of VL
determined by the COBAS AmpliPrep/TaqMan assays and the in-house assays used.
%K COBAS AmpliPrep/TaqMan
%K In-House PCR Assays
%K HIV-1 Non-B
%U http://www.oalib.com/paper/3143518