%0 Journal Article
%T Effects of Light Emitting Diode  in Erythrocytes
%A Hennes Gentil de Ara¨˛jo
%A Patricia Froes Meyer
%A Mario Bernardo Filho
%A ¨Śric Heleno Freire Frederico
%A SebastiŁżo David dos Santos Filho
%A Marcus Vinicius de Mello Pinto
%J Modern Research in Inflammation
%P 122-127
%@ 2169-9690
%D 2014
%I Scientific Research Publishing
%R 10.4236/mri.2014.33015
%X <p class=\"MsoNormal\" style=\"text-align:justify;\">
	<span lang=\"EN-US\" style=\"font-size:10pt;font-family:'}', serif;\"><span style=\"font-size:12px;font-family:Verdana;\">The Light Emitting Diode
(LED) is a phototherapy equipment, poorly studied, which is able of acting on
blood level and it has frequently appeared in clinical practice of
physiotherapy to aid on skin rejuvenation, control inflammation and wound
healing. The aim of the study was to evaluate the effects of LED on red blood
cells through the spectrophotometer. This is an experimental study with a
sample of 5 male Wistar rats weighting between 200 g and 350 g, randomly
selected, anesthetized and 4 ml of blood was collected by cardiac puncture. The
blood collected was divided into four groups, one control and three treated,
these individually irradiated by blue LED (420 - 490 nm), green LED (515 - 570
nm) and red LED (620 - 680 nm) at an output of 3 watts, for 10 minutes and 5 cm
apart from the surface of the blood, then it was analyzed by a
spectrophotometer at 540 nm. The results were submitted to Anova and Post-roch
Turkey (</span><i><span style=\"font-size:12px;font-family:Verdana;\">p</span></i><span class=\"apple-converted-space\" style=\"font-size:12px;font-family:Verdana;\"> </span><span style=\"font-size:12px;font-family:Verdana;\">&lt; 0.05). All
wavelengths tested</span><span class=\"apple-converted-space\" style=\"font-size:12px;font-family:Verdana;\"> </span><span style=\"font-size:12px;font-family:Verdana;\">produced
hemolysis greater than the control in hypotonic solutions of NaCl (range 0.02
to 0.06 M) (</span><i><span style=\"font-size:12px;font-family:Verdana;\">p</span></i><span class=\"apple-converted-space\" style=\"font-size:12px;font-family:Verdana;\"> </span><span style=\"font-size:12px;font-family:Verdana;\">&lt; 0.01).
Only the band between 515 - 570 nm provides a higher hemolysis (range 0.06 to
0.1) when compared to the control group (</span><i><span style=\"font-size:12px;font-family:Verdana;\">p</span></i><span class=\"apple-converted-space\" style=\"font-size:12px;font-family:Verdana;\"> </span><span style=\"font-size:12px;font-family:Verdana;\">&lt; 0.05). The irradiation of blood
through the LED therapy was able to weaken the cell membrane of the red blood
cells. It is likely that hemolysis does not bring harm the body, because as it
is a local therapy, the amount of hemolysis that can be caused is small and it
will induce the reproduction of new red blood cells, thus improving their
physiological functions.</span></span> 
</p>
%K LED
%K Blood
%K Spectrophotometer
%U http://www.scirp.org/journal/PaperInformation.aspx?PaperID=49001