%0 Journal Article
%T Lidocaine-Induced Cell Growth of Human Gingival Fibroblasts. Role of Na<sup>+</sup>-K<sup>+</sup>-ATPase and PKC Activities
%A Emmanuel Quinteros Villarruel
%A Betina Orman
%A Enri Borda
%J Pharmacology & Pharmacy
%P 796-805
%@ 2157-9431
%D 2014
%I Scientific Research Publishing
%R 10.4236/pp.2014.58090
%X <p style=\"text-align:justify;\">
	<i><span style=\"font-size:10pt;line-height:1.5;font-family:Verdana;\">Background</span></i><span style=\"font-size:10.0pt;line-height:97%;font-family:;\" \"=\"\"><span style=\"font-family:Verdana;line-height:1.5;\">:
Evidences have shown that local anaesthetics are clinically useful compounds that
exert a pharmacological effect by blocking nerve impulse propagation and also it
is able to provoke proliferation and cell growth. </span><i><span style=\"font-family:Verdana;line-height:1.5;\">Aims</span></i><span style=\"font-family:Verdana;line-height:1.5;\">: The aim of this study was to investigate the proliferation and
cell growth capacity of lidocaine on human gingival fibroblast cells and the different
signal pathways involved in its effect. </span><i><span style=\"font-family:Verdana;line-height:1.5;\">Method</span></i><span style=\"font-family:Verdana;line-height:1.5;\">:
For this purpose </span><i><span style=\"font-family:Verdana;line-height:1.5;\">in</span></i> <i><span style=\"font-family:Verdana;line-height:1.5;\">vitro</span></i><span style=\"font-family:Verdana;line-height:1.5;\"> cultures of human gingival fibroblasts
were assayed and the effects of lidocaine on proliferation and cell DNA synthesis,
Na</span><sup><span style=\"font-family:Verdana;line-height:1.5;\">+</span></sup><span style=\"font-family:Verdana;line-height:1.5;\">-K</span><sup><span style=\"font-family:Verdana;line-height:1.5;\">+</span></sup><span style=\"font-family:Verdana;line-height:1.5;\">-ATPase and PKC activities and K</span><sup><span style=\"font-family:Verdana;line-height:1.5;\">+</span></sup><span style=\"font-family:Verdana;line-height:1.5;\"> efflux
were also evaluated. </span><i><span style=\"font-family:Verdana;line-height:1.5;\">Results</span></i><span style=\"font-family:Verdana;line-height:1.5;\">: Lidocaine
stimulated in a concentration-dependent manner proliferation and cell growth of
human gingival cells and the mechanism involve an increment in Na</span><sup><span style=\"font-family:Verdana;line-height:1.5;\">+</span></sup><span style=\"font-family:Verdana;line-height:1.5;\">-K</span><sup><span style=\"font-family:Verdana;line-height:1.5;\">+</span></sup><span style=\"font-family:Verdana;line-height:1.5;\">-ATPase
and PKC activities, which led to an increase in K</span><sup><span style=\"font-family:Verdana;line-height:1.5;\">+</span></sup><span style=\"font-family:Verdana;line-height:1.5;\"> release. All of these
effects were blocked by tetrodotoxin, ouabain and calphostin C. In addition, PMA
(activator of PKC)
%K Lidocaine
%K DNA Synthesis
%K Human Gingival Fibroblasts
%K Na+-K+-ATPase
%K PKC
%U http://www.scirp.org/journal/PaperInformation.aspx?PaperID=47686