%0 Journal Article
%T Induced Transcriptional Expression of  <i>Bacillus subtilis</i> Amino Acid Permease  <i>yvbW</i> in Response to Leucine Limitation
%A Sean M. Rollins
%J Advances in Microbiology
%P 484-492
%@ 2165-3410
%D 2014
%I Scientific Research Publishing
%R 10.4236/aim.2014.48053
%X <p>
	<span style=\"font-family:&quot;\"><span style=\"line-height:2;font-family:Verdana;\">T box sequences have
been identified upstream of a large number of uncharacterized genes such as
transporters in bacterial genomes. Expression of each T box family gene is
induced by limitation for a specific amino acid. T box family genes contain an
untranslated leader region containing a factor-independent transcriptional
terminator upstream of the structural genes. The anticodon of uncharged tRNA
base-pairs with the leader mRNA at a codon referred to as the specifier sequence,
inducing formation of an alternative antiterminator structure, allowing
expression of the structural genes.</span><span style=\"line-height:2;font-family:Verdana;\"> There
are several additional conserved primary sequence</span><span style=\"line-height:2;font-family:Verdana;\"> and secondary structural
elements. Analysis of these elements can be used to predict the identity of the
specifier codon and the amino acid signal.&nbsp;</span><i><span style=\"line-height:2;font-family:Verdana;\">Bacillus subtilis</span></i><i> </i><span style=\"line-height:2;font-family:Verdana;\">hypothetical
amino acid permease, </span><i><span style=\"line-height:2;font-family:Verdana;\">yvbW</span></i><span style=\"line-height:2;font-family:Verdana;\">, was analyzed
as an example of this type of transcriptional regulatory prediction suggesting expression
in response to leucine limitation. Expression was induced up to 130-fold in
response to leucine limitation, utilizing a </span><i><span style=\"line-height:2;font-family:Verdana;\">yvbW-lacZ</span></i><span style=\"line-height:2;font-family:Verdana;\"> transcriptional fusion. These data suggest that hypothetical amino acid
permease YvbW may participate in leucine metabolism. A </span><i><span style=\"line-height:2;font-family:Verdana;\">yvbW</span></i><span style=\"line-height:2;font-family:Verdana;\"> knockout strain was generated, although the substrate
specificity for the putative amino acid permease was not identified.</span></span> 
</p>
%K T Box
%K Antitermination
%K Riboswitch
%K Amino Acid Permease
%K YvbW
%U http://www.scirp.org/journal/PaperInformation.aspx?PaperID=47407