%0 Journal Article
%T Rapid and Sensitive CZE Method for Quality Control Analysis of Pharmaceuticals Containing Pseudoephedrine, Triprolidine and Paracetamol
%A Sonia Di Berardino
%A Renata Jasionowska
%J American Journal of Analytical Chemistry
%P 613-619
%@ 2156-8278
%D 2014
%I Scientific Research Publishing
%R 10.4236/ajac.2014.59069
%X A rapid and sensitive CZE (Capillary Zone Electrophoresis) method for pharmaceutical analysis was developed and fully validated. The active compounds: Pseudoephedrine hydrochloride (PSE), Triprolidine hydrochloride (TRI) and Paracetamol (PAR) were separated and quantitatively determined using the tris-borate 30 mM buffer at pH = 9.0 as a Background Electrolyte (BGE). The electrophoretic separation was carried out at 25 kV in an unmodified fused silica capillary of I.D. = 50 ¦Ìm with a ¡°bubble-cell¡± for UV detection at 210 nm and 25°C. The separation was reached in about 3 min. After calibration the method was applied for analysis of three commercially available pharmaceutical preparations. The repeatability (RSD%) of migration time (tm) was ranging between 0.47% and 0.90% and of peak areas (A) between 0.63% and 3.64%. The Limit of Detection (LOD) values was of 0.19 ¦Ìg/mL, 0.31 ¦Ìg/mL and 0.08 ¦Ìg/mL for respectively PSE, TRI and PAR. The results obtained in this study showed that the proposed method was useful in routinely analysis of pharmaceuticals.
%K Capillary Zone Electrophoresis
%K Triprolidine
%K Pseudoephedrine
%K Paracetamol
%K Pharmaceutical Analysis
%K Validation
%U http://www.scirp.org/journal/PaperInformation.aspx?PaperID=47156