%0 Journal Article
%T Comparative Analysis of Genetic Diversity among Cultivated Pigeonpea (<i>Cajanus cajan</i> (L) Millsp.) and Its Wild Relatives (<i>C. albicans</i> and <i>C. lineatus</i>) Using Randomly Amplified Polymorphic DNA (RAPD) and Inter Simple Sequence Repeat (ISSR) Fingerprinting
%A Kusum Yadav
%A Sanjay Kumar Yadav
%A Anurag Yadav
%A Veda Prakash Pandey
%A Upendra Nath Dwivedi
%J American Journal of Plant Sciences
%P 1665-1678
%@ 2158-2750
%D 2014
%I Scientific Research Publishing
%R 10.4236/ajps.2014.511181
%X <p style=\"text-align:justify;\">
	<span style=\"font-size:10.0pt;font-family:;\" \"=\"\"><span style=\"font-family:Verdana;\">Genetic
relationships of 16 cultivars of pigeonpea (</span><i><span style=\"font-family:Verdana;\">Cajanus
cajan</span></i><span style=\"font-family:Verdana;\"> (L) Millsp.) and its two wild relatives (</span><i><span style=\"font-family:Verdana;\">C. albicans</span></i><span style=\"font-family:Verdana;\"> and </span><i><span style=\"font-family:Verdana;\">C. lineatus</span></i><span style=\"font-family:Verdana;\">)
from different parts of the India were analysed using 22 random amplified
polymorphic DNAs (RAPDs) primers and 10 inter simple sequence repeats (ISSRs)
primers. Twenty two RAPD primers yielded 151 polymorphic markers (71.2%) with
an average of 6.8 polymorphic band/primer. Cluster analysis based on these 151
RAPD markers revealed relatively low level (0.434 - 0.714) of genetic diversity
among cultivars and high level of diversity between cultivars and wild
relatives. Ten ISSR primers produced 100 bands across 16 cultivars and its wild </span><span style=\"font-family:Verdana;\">relatives out of which 93 (93%) were
polymorphic with an average of 9.3 polymorphic band/primer</span><span style=\"font-family:Verdana;\">. Cluster
analysis based on these 93 ISSR markers also revealed relatively higher level (0.328
- 0.827) of genetic diversity among cultivars as compared to RAPD markers. The
polymorphic markers obtained by both RAPD and ISSR primers were pooled and the
genetic diversity analysis based on these 244 markers was analysed. Jaccard¡¯s
similarity coefficient obtained by pooled data revealed very narrow range (0.477
- 0.720) among cultivated and high range between cultivated and wild species </span><i><span style=\"font-family:Verdana;\">C. albicans</span></i><span style=\"font-family:Verdana;\"> (0.240 - 0.331) and </span><i><span style=\"font-family:Verdana;\">C. lineatus</span></i><span style=\"font-family:Verdana;\"> (0.163 - 0.193). In the
UPGMA based dendrogram the 16 cultivars were grouped into three distinct
clusters. Cluster I contained two genotypes, cluster II had six and cluster III
had eight genotypes. Principal components analysis (PCA) also resulted in
similar pattern as that of UPGMA based analysis. The first three PCs contributed
56.26%, 5.71% and 4.97% of variation, respectively, with cumulative variation
of the first three PCs was 66.96%. Both the markers and the combined data
revealed similar pattern with narrow diversity among cultivars and higher
diversity between cultivars and wild one, but
%K Genetic Diversity
%K RAPD
%K ISSR
%K Pigeonpea
%U http://www.scirp.org/journal/PaperInformation.aspx?PaperID=46093