%0 Journal Article
%T Expression of Telomerase Reverse Transcriptase during the Malignant Transformation of Cadmium-Induced Cells
%A Min Wang
%A Yixiong Lei
%J Journal of Geoscience and Environment Protection
%P 129-133
%@ 2327-4344
%D 2014
%I Scientific Research Publishing
%R 10.4236/gep.2014.22018
%X The objective of the present study was to investigate human telomerase reverse transcriptase (hTERT) mRNA and protein expressions during the cadmium chloride-induced malignant transformation of human bronchial epithelial (16HBE) cells. Fluorescence quantitative PCR (FQ-PCR) and Western blot analyses were performed to detect the hTERT mRNA and protein expressions in normal 16HBE cells, cadmium chloride-transformed 16HBE cells, and tumorigenic cells from nude mice inoculated with cadmium chloride-transformed 16HBE cells. Under the inner standard of GAPDH, the hTERT mRNA expression was significantly higher at different stages of malignant transformation (cadmium chloride-transformed 16HBE cells at passages 15 and 35 and tumorigenic cells from nude mice) than in normal 16HBE cells, and increased with the development of malignancy (P < 0.01). In addition, hTERT protein expression increased with the development of malignancy. These findings demonstrate that hTERT expression is related to cadmium chlorideinduced malignant transformation. Cadmium chloride-induced malignant transformation is involved in changes in the hTERT activity, and might be an early event in cadmium chloride-induced malignant transformation.
%K Cadmium Chloride
%K Human Bronchial Epithelial Cells
%K Malignant Transformation
%K Telomerase Reverse Transcriptase
%U http://www.scirp.org/journal/PaperInformation.aspx?PaperID=45028