%0 Journal Article
%T Development of a Rapid and Efficient Liquid Chromatography Method for Determination of Gibberellin A4 in Plant Tissue, with Solid Phase Extraction for Purification and  Quantification
%A Julia Medrano Mac¨ªas
%A Rahim Foroughbakhch Pournavab
%A Manuel Humberto Reyes-Vald¨¦s
%A Adalberto Benavides-Mendoza
%J American Journal of Plant Sciences
%P 573-583
%@ 2158-2750
%D 2014
%I Scientific Research Publishing
%R 10.4236/ajps.2014.55072
%X <p style=\"text-align:justify;\">
	<span style=\"font-size:10.0pt;font-family:;\" \"=\"\"><span style=\"font-family:Verdana;font-size:12px;\">A new, rapid and efficient reverse phase
Liquid Chromatography (RP-LC) method was developed for determination of
Gibberellin A4 (GA4) in samples of flower stalk of </span><i><span style=\"font-family:Verdana;font-size:12px;\">Dasylirion cedrosanum </span></i><span style=\"font-family:Verdana;font-size:12px;\">and
vegetative tissue of </span><i><span style=\"font-family:Verdana;font-size:12px;\">Epithelantha
micromeris</span></i><span style=\"font-family:Verdana;font-size:12px;\">. Purification of GA4 was carried out by solid phase extraction
(SPE), in </span><i><span style=\"font-family:Verdana;font-size:12px;\">Epithelantha micromeris. </span></i><span style=\"font-family:Verdana;font-size:12px;\">In
the chromatography method was obtaining a retention time of 2.1 min, using
Hypersil GOLD C-18 column (100 ¡Á</span></span><span style=\"font-size:10.0pt;font-family:;\" \"=\"\"> </span><span style=\"font-size:12px;font-family:Verdana;\">4.6 mm dim and size particle 5 ¦Ì), mobile
phase 50/50 acetonitrile/water and a flow 1.0 ml/min. Detection was carried out
by a UV detector set at 205 nm, and a quantization limit of 0.4 mg/L. The
obtained correlation coefficient was 0.995.</span> 
</p>
%K &lt
%K i&gt
%K Dasyrilon cedrosanum&lt
%K /i&gt
%K &lt
%K i&gt
%K Epithelantha micromeris&lt
%K /i&gt
%K Phytohormone Separation
%K Plant Growth  Regulators
%K Reverse Phase Liquid Chromatography
%K Solid Phase Extraction
%U http://www.scirp.org/journal/PaperInformation.aspx?PaperID=43615