%0 Journal Article
%T Protein Bodies in Cotyledon Cells Exhibit Differential Patterns of Legumin-Like Proteins Mobilization during Seedling Germinating States
%A Jose C. Jimenez-Lopez
%A Maria C. Hernandez-Soriano
%J American Journal of Plant Sciences
%P 2444-2454
%@ 2158-2750
%D 2013
%I Scientific Research Publishing
%R 10.4236/ajps.2013.412304
%X <p class=\"MsoNormal\">
	<span lang=\"EN-US\"><span style=\"font-family:Verdana;\">Olive (</span><i><span style=\"font-family:Verdana;\">Olea europaea</span></i><span style=\"font-family:Verdana;\"> L.) tree is one of the most extensive and important agricultural crop in Mediterranean countries due to its beneficial health and nutritional properties and its high economic value. Currently, olive tree constitutes the sixth most important cultivated plant in the world, spreading from the Mediterranean region of origin to new production areas such as Australia, South and North America and South Africa. However, the mobilization processes of storage materials </span><i><span style=\"font-family:Verdana;\">i.e</span></i><span style=\"font-family:Verdana;\">. reserve proteins during seed germination, which are largely involved in essential physiological process including plant growth and development, remain poorly understood.</span></span><span lang=\"EN-US\"> </span><span style=\"font-family:Verdana;\" lang=\"EN-US\">Morphometric and immunohistochemistry analy</span><span style=\"font-family:Verdana;\" lang=\"EN-US\">ses of protein bodies contained in olive seed storage tissues, cotyledon and endosperm, were performed by using dif</span><span style=\"font-family:Verdana;\" lang=\"EN-US\">ferent microscopy techniques, including light (bright-field and fluorescence) microscopy and transmission electron mi</span><span style=\"font-family:Verdana;\" lang=\"EN-US\">croscopy. Furthermore, we used legumin-like proteins (11S-type globulins) as a molecular marker to study the mobili</span><span style=\"font-family:Verdana;\" lang=\"EN-US\">zation of reserve proteins from PBs of cotyledons at germinating seedling stages by using immunofluorescence assays.</span><span lang=\"EN-US\"> </span><span style=\"font-family:Verdana;\" lang=\"EN-US\">Results demonstrated that cotyledon and endosperm are characterized by distinct PBs populations containing legu</span><span style=\"font-family:Verdana;\" lang=\"EN-US\">min-like proteins, distinctly discriminated by the number of PBs per cell and tissue, size, immunofluorescence and his</span><span style=\"font-family:Verdana;\" lang=\"EN-US\">tochemical staining. These features reflect differential PBs biogenesis during development and maturation processes in olive seed tissues endosperm and cotyledon, in relation to proteins (polypeptides) final composition, SSPs processing and/or packaging during seed maturation. Three different mobilization patterns of legumin-like proteins were identified for the first time in cotyledon PBs
%K 11S Globulins
%K Cotyledon
%K Endosperm
%K &lt
%K i&gt
%K In Vitro&lt
%K /i&gt
%K Germination
%K Legumin-Like Proteins
%K &lt
%K i&gt
%K Olea europaea&lt
%K /i&gt
%K L.
%K Protein Bodies
%K Seed Proteins Mobilization
%U http://www.scirp.org/journal/PaperInformation.aspx?PaperID=41296