%0 Journal Article %T Expanded Genetic Codes in Next Generation Sequencing Enable Decontamination and Mitochondrial Enrichment %A Kevin J. McKernan %A Jessica Spangler %A Lei Zhang %A Vasisht Tadigotla %A Stephen McLaughlin %A Jason Warner %A Amir Zare %A Richard G. Boles %J PLOS ONE %D 2014 %I Public Library of Science (PLoS) %R 10.1371/journal.pone.0096492 %X We have developed a PCR method, coined D¨¦j¨¤ vu PCR, that utilizes six nucleotides in PCR with two methyl specific restriction enzymes that respectively digest these additional nucleotides. Use of this enzyme-and-nucleotide combination enables what we term a ˇ°DNA diodeˇ±, where DNA can advance in a laboratory in only one direction and cannot feedback into upstream assays. Here we describe aspects of this method that enable consecutive amplification with the introduction of a 5th and 6th base while simultaneously providing methylation dependent mitochondrial DNA enrichment. These additional nucleotides enable a novel DNA decontamination technique that generates ephemeral and easy to decontaminate DNA. %U http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0096492