%0 Journal Article
%T Oleic, Linoleic and Linolenic Acids Increase ROS Production by Fibroblasts via NADPH Oxidase Activation
%A Elaine Hatanaka
%A Alexandre Dermargos
%A Aparecida Emiko Hirata
%A Marco Aur¨¦lio Ramirez Vinolo
%A Angelo Rafael Carpinelli
%A Philip Newsholme
%A Hugo Aguirre Armelin
%A Rui Curi
%J PLOS ONE
%D 2013
%I Public Library of Science (PLoS)
%R 10.1371/journal.pone.0058626
%X The effect of oleic, linoleic and ¦Ã-linolenic acids on ROS production by 3T3 Swiss and Rat 1 fibroblasts was investigated. Using lucigenin-amplified chemiluminescence, a dose-dependent increase in extracellular superoxide levels was observed during the treatment of fibroblasts with oleic, linoleic and ¦Ã-linolenic acids. ROS production was dependent on the addition of ¦Â-NADH or NADPH to the medium. Diphenyleneiodonium inhibited the effect of oleic, linoleic and ¦Ã-linolenic acids on fibroblast superoxide release by 79%, 92% and 82%, respectively. Increased levels of p47phox phosphorylation due to fatty acid treatment were detected by Western blotting analyses of fibroblast proteins. Increased p47phox mRNA expression was observed using real-time PCR. The rank order for the fatty acid stimulation of the fibroblast oxidative burst was as follows: ¦Ã-linolenic > linoleic > oleic. In conclusion, oleic, linoleic and ¦Ã-linolenic acids stimulated ROS production via activation of the NADPH oxidase enzyme complex in fibroblasts.
%U http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0058626