%0 Journal Article
%T Dominance of Enterobacteria among Histamine-Producing Bacteria Isolated from Indian Mackerel
%A Meena Tembhurne
%A Anita Ghag
%A Hirekudel Sanathkumar
%A Binaya Bhusan Nayak
%J Advances in Microbiology
%P 537-542
%@ 2165-3410
%D 2013
%I Scientific Research Publishing
%R 10.4236/aim.2013.37072
%X <p class=\"MsoNormal\" style=\"text-align:justify;\">
	<span style=\"font-family:Verdana;\" lang=\"EN-US\">Histamine fish poisoning (HFP) is a major illness occurring
throughout the world due </span><span style=\"font-family:Verdana;\" lang=\"EN-US\">to </span><span style=\"font-family:Verdana;\" lang=\"EN-US\">the consumption of quality </span><span style=\"font-family:Verdana;\" lang=\"EN-US\">of </span><span lang=\"EN-US\"><span style=\"font-family:Verdana;\">deteriorated
fish containing pre-formed histamine from bacterial activities. In the study
reported here, the histamine-pro</span><a name=\"_GoBack\"></a><span style=\"font-family:Verdana;\">ducing bacteria were
isolated from the muscle, gills and the gut of 19 samples of Indian mackerel (</span><i><span style=\"font-family:Verdana;\">Rastrelliger kanagurta</span></i><span style=\"font-family:Verdana;\">) from Mumbai,
India. The isolates from modified Niven¡¯s medium (MNM) were confirmed for their
ability to produce histamine </span></span><span style=\"font-family:Verdana;\" lang=\"EN-US\">by </span><span lang=\"EN-US\"><span style=\"font-family:Verdana;\">using 4 different pH-indicator media,
followed by HPLC analyses. Out of 202 isolates, 63 isolates produced
considerable amounts of histamine on at least 3 out of 4 media used in this
study. The histamine formers were identified by biochemical tests followed by
sequencing of their </span><i><span style=\"font-family:Verdana;\">16SrDNA</span></i><span style=\"font-family:Verdana;\"> gene,
which showed that 89% of the isolates belonged to 13 different genera of the
family Enterobacteriaceae. The non-enterobacterial histamine-producing bacteria
belonged to the genera </span><i><span style=\"font-family:Verdana;\">Staphylococcus</span></i><span style=\"font-family:Verdana;\">, </span><i><span style=\"font-family:Verdana;\">Alkaligenes</span></i><span style=\"font-family:Verdana;\">, </span><i><span style=\"font-family:Verdana;\">Shewanella</span></i><span style=\"font-family:Verdana;\"> and </span><i><span style=\"font-family:Verdana;\">Psychrobacter</span></i><span style=\"font-family:Verdana;\">.</span></span><span lang=\"EN-US\"><o:p></o:p></span> 
</p>
<p class=\"MsoNormal\" style=\"text-align:justify;\">
	<span style=\"font-family:Verdana;\" lang=\"EN-US\"> </span> 
</p>
%K Histamine
%K Scombroid Fish
%K Enterobacteria
%K &lt
%K i&gt
%K 16SrDNA&lt
%K /i&gt
%U http://www.scirp.org/journal/PaperInformation.aspx?PaperID=40134