%0 Journal Article
%T Neural differentiation of allogenic mixed-cultured rat bone mesenchymal stem cells
%A Jun Zhou
%A Huilin Yang
%A Jiannong Cen
%J Journal of Biomedical Science and Engineering
%P 466-472
%@ 1937-688X
%D 2013
%I Scientific Research Publishing
%R 10.4236/jbise.2013.64058
%X <p>
	<span style=\"font-size:10.0pt;\"><span style=\"font-family:Verdana;\">Many
studies showed that bone mesenchymal stem cells (BMSCs) can transdifferentiate
to neural cell </span><i><span style=\"font-family:Verdana;\">in vitro</span></i><span style=\"font-family:Verdana;\">. The purpose
of this study was to investigate the mixed-culture of allogenic rat BMSCs </span><i><span style=\"font-family:Verdana;\">in vitro</span></i><span style=\"font-family:Verdana;\">, and to perform neural
differentiation potential characterization. Rat BMSCs were isolated and
cultured by plastic adherence and density gradient centrifugation respectively,
and the 3rd passage cells were harvested and mixed-cultured with same
concentration. The second passage cells of the mixed-cultured cells were
obtained to perform Wright-Gemsa staining for morphological observation, to
identify the surface marker of CD29, CD45, CD90 by flow cytometry and induced
to differentiate into neural cell, then performed immunocytochemistry of
Nestin, NSE and GFAP. Results showed that after Wright-Giemsa staining, the
mixed-cultured cells displayed typical spindle-shape, presented layered and
whirlpool-like growth; and the </span><span style=\"font-family:Verdana;\">mixed-cultured
cells were positive for CD29 and</span><span style=\"font-family:Verdana;\"> CD90, but negative for CD45 by flow
cytometry. After induction, the mixed-cultured cells appeared morphological
changes of neuron and glial cell, and were positive expression of Nestin,
neuron specific enolase (NSE) and glial fibrillary acidic protein (GFAP). The
results demonstrated that the mixed-cultured allogenic rat BMSCs can be
induced into neural cell such as neuron and glial cell </span><i><span style=\"font-family:Verdana;\">in vitro</span></i><span style=\"font-family:Verdana;\">.</span></span> 
</p>
%K Bone Mesenchymal Stem Cells
%K Neural  Differentiation
%K Nestin
%K Neuron Specific Enolase
%K Glial Fibrillary Acidic Protein
%U http://www.scirp.org/journal/PaperInformation.aspx?PaperID=30506