%0 Journal Article
%T Titrimetric, spectrophotometric and kinetic methods for the assay of atenolol using bromate每bromide and methyl orange
%A KANAKAPURA BASAVAIAH
%A UMAKANTHAPPA CHANDRASHEKAR
%A PAREGOWDA NAGEGOWDA
%J Journal of the Serbian Chemical Society
%D 2006
%I Serbian Chemical Society
%X Three new methods have been developed for the determination of atenolol in bulk drug and in tablet formulation. The methods are based on the oxidation每bromination reaction of the drug by bromine, generated in situ by the action of acid on a bromate每bromide mixture. In the titrimetric method, the drug is treated with a known excess of bromate每bromide mixture in hydrochloric acid medium, followed by the determination of the unreacted bromine iodometrically. The spectrophotometric method involves the addition of a measured excess of bromate每bromide reagent in hydrochloric acid medium to atenolol, and after ensuring the reaction had gone to completion, the unreacted bromine is treated with a fixed amount of methyl orange, and absorbance measured at 520 nm. The absorbance was found to increase linearly with increasing concentration of atenolol. The kinetic method depends on the existence of a linear relationship between the concentration of the drug and the time of the oxidation每bromination reaction, as indicated by the bleaching of methyl orange acid colour. The working conditions were optimized. The titrimetric method is based on a 1:1 reaction stoichiometry (atenolol:bromate) and is applicable over the 3每20 mg range. The spectrophotometric method permits micro determination of the drug (0.5每4.0 mg ml-1)with an apparentmolar absorptivity of 4.13x104 lmol-1 cm-1 and detection limit of 0.07 mg ml-1. The kinetic method is applicable in the concentration range 5每25 mg ml-1 with a detection limit of 3.72 mg ml-1. The proposed methods were successfully applied to the determination of atenolol in tablet preparations with mean recoveries of 97.63 to 101.78 %. The reliability of the assay was established by parallel determination by the reference method and by recovery studies using the standard addition technique.
%K atenolol
%K determination
%K titrimetry
%K spectrophotometry
%K kinetics
%K bromate每bromide reagent
%K methyl orange
%K tablets
%U http://www.shd.org.yu/HtDocs/SHD/Vol71/No5/JSCS_V71_No5-11.pdf