%0 Journal Article
%T Spectral properties of LH2 exhibit very similar even when heterologously express LH2 with <i>¦Â</i>-subunit fusion protein in <i>Rhodobacter</i> <i>sphaeroides</i>
%A Zhiping Zhao
%A Xin Nie
%A Zongli Hu
%A Guoping Chen
%A Zaixin Li
%A Zhi Zhang
%J Advances in Biological Chemistry
%P 101-107
%@ 2162-2191
%D 2013
%I Scientific Research Publishing
%R 10.4236/abc.2013.31013
%X <p style=\"text-align:justify;\">
	<span style=\"font-size:10.0pt;\"><span style=\"font-size:12px;\">Interactions between the light-harvesting subunits
and the non-covalently bound photopigments attribute considerably to the
spectral properties of photosynthetic bacteria light-harvesting complexes. In
our previous studies, we have constructed a novel </span><i><span style=\"font-size:12px;\">Rhodobacter sphaeroides</span></i><span style=\"font-size:12px;\"> expression system. In the present study,
we focus on the spectral properties of LH2 when heterologously express LH2 with </span><i><span style=\"font-size:12px;\">¦Â</span></i><span style=\"font-size:12px;\">-subunit- GFP fusion protein in</span><i><span style=\"font-size:12px;\"> Rb. sphaeroides</span></i><span style=\"font-size:12px;\">. Near infra-red
spectrum of LH2 remained nearly unchanged as measured by spectroscopy.
Fluorescence spectrum suggested that the LH2 with </span><i><span style=\"font-size:12px;\">¦Â</span></i><span style=\"font-size:12px;\">-subunit-GFP fusion protein complexes still possessed normal
activity in energy transfer. However, photopigments contents were
significantly decreased to a very low lev</span><span style=\"font-size:12px;\">el in the LH2
with </span><i><span style=\"font-size:12px;\">¦Â</span></i><span style=\"font-size:12px;\">-subunit-GFP fusion protein
complexes compared to that of LH2. FT-IR spectra indicated that interactions
between photopigments and LH2 </span><i><span style=\"font-size:12px;\">¦Á</span></i><span style=\"font-size:12px;\">/</span><i><span style=\"font-size:12px;\">¦Â</span></i><span style=\"font-size:12px;\">- subunits appeared not to be changed.
It was concluded that the LH2 spectral properties exhibited very similar even
when heterologously expressed LH2 </span></span><span style=\"font-size:12px;\">b</span><span style=\"font-size:10.0pt;\"><span style=\"font-size:12px;\">-subunit
fusion protein in </span><i><span style=\"font-size:12px;\">Rb. sphaeroides</span></i><span style=\"font-size:12px;\">.
Our present study may supply a new insight into better understand the interactions
between light-harvesting subunits and photopigments and bacterial photosynthesis
and promote the development of the novel </span><i><span style=\"font-size:12px;\">Rb.
sphaeroides</span></i><span style=\"font-size:12px;\"> expression system.</span></span> 
</p>
%K LH2
%K Spectral Property
%K FT-IR
%K Photopigment
%U http://www.scirp.org/journal/PaperInformation.aspx?PaperID=28113