%0 Journal Article %T The Effect of Resveratrol on Bone Status in Rats with Bile Duct Obstruction %A Helena Zivna %A Stanislav Micuda %A Eva Brcakova-Dolezelova %A Pavel Zivny %J Open Journal of Endocrine and Metabolic Diseases %P 46-51 %@ 2165-7432 %D 2013 %I Scientific Research Publishing %R 10.4236/ojemd.2013.31007 %X

Objective: We studied the influence of bile duct obstruction (BDO) after 28 days on bone metabolism status and their modification by resveratrol in male Wistar rats. Methods: The rats were divided into 3 groups: Sham group with laparotomy and vehiculum application, group BDO¡ªbile duct obstruction and vehiculum application, and group RBDO¡ªbile duct obstruction and resveratrol application (10 mg/kg dose of resveratrol orally once daily). The bone mineral density (BMD; g/cm2) and body composition were measured by dual energy X-ray absorptiometry. The physiccal strenght of femur was examined by controlled break biomechanical testing. The osteocalcin, procollagen type I N-terminal propeptide (PINP) and carboxy-terminal collagen crosslinks (CTX) were analysed by EIA in the bone tissue homogenate. Results: The total BMD was lower in group BDO (0.176 ¡À 0.005; p = 0.01) and R-BDO (0.181 ¡À 0.004; p = 0.052) vs Sham (0.209 ¡À 0.003). BMD of femur diaphysis in BDO group decreased significantly (p = 0.01) but not in R-BDO animals (p = 0.052). Body fat (g, median) was lower in BDO (19.0) and R-BDO (26.0) than in Sham (35.0). The force needed for fracture of femurs (N) significantly decreased in BDO (154 ¡À 6) and R-BDO (151 ¡À 13) vs Sham group (208 ¡À 7). The force needed for neck fracture decreased in BDO (105 ¡À 3) and R-BDO (115 ¡À 8) vs Sham (135 ¡À 9). The osteocalcin decrease in group R-BDO (2.9 ¡À 0.1) vs Sham (3.7 ¡À 0.4). Higher PINP/CTX were in BDO (20 ¡À %K Bone Metabolism %K Rat %K Resveratrol %K Bile Duct Obstruction %U http://www.scirp.org/journal/PaperInformation.aspx?PaperID=28031