%0 Journal Article
%T Adhesion of <i>Gallibacterium anatis</i> to Chicken Oropharyngeal Epithelial Cells and the Identification of Putative Fimbriae
%A M¨®nica L. Salgado Lucio
%A Sergio Vaca
%A Candelario V¨¢zquez
%A Edgar Zenteno
%A Ismael Rea
%A V¨ªctor M. P¨¦rez-M¨¢rquez
%A Erasmo Negrete-Abascal
%J Advances in Microbiology
%P 505-510
%@ 2165-3410
%D 2012
%I Scientific Research Publishing
%R 10.4236/aim.2012.24064
%X Microbial infections are typically initiated by the colonization of tissues by a specific mechanism that promotes adherence to host cells or tissues. In this work, we characterized the ability of <i>Gallibacterium anatis</i> F149<sup>T</sup> to express fimbriae that may be involved in mucosal attachment. Using transmission electron microscopy, the fimbriae-like structures could be observed on the surface of negatively stained <i>G. anatis</i> F149<sup>T</sup>, and these structures were further visualized after being released by physical shaking. When the fimbriae-like structures were separated by SDS-PAGE, the proteins comprising them were isolated and sized at 13 and 25 kDa. <i>G. anatis</i> F149<sup>T</sup> was able to adhere to chicken oropharyngeal epithelial cells. Adhesion could be completely inhibited by pretreatment of the bacterial cells with trypsin, whereas 25% inhibition was attained after pretreatment with an antiserum against the 13 kDa protein. We demonstrated by immuno-gold electron microscopy that the antibodies from the antiserum were specifically associated with the fimbria-like structures on <i>G. anatis</i>. These results indicated that <i>G. anatis</i> F149<sup>T</sup> expresses fimbriae that contribute to its adhesion to chicken oropharyngeal epithelial cells and may be important for colonization of the upper respiratory tract.
%K &lt
%K i&gt
%K G. anatis&lt
%K /i&gt
%K Adhesion
%K Fimbria
%K Pili
%U http://www.scirp.org/journal/PaperInformation.aspx?PaperID=25856