%0 Journal Article
%T Alternative expression of TCR¦Æ related genes in patients with chronic myeloid leukemia
%A Zha Xianfeng
%A Yan Xiaojuan
%A Shen Qi
%A Zhang Yuping
%J Journal of Hematology & Oncology
%D 2012
%I BioMed Central
%R 10.1186/1756-8722-5-74
%X A previous study has demonstrated a significant decrease in the TCR¦Æ gene expression level in chronic myeloid leukemia (CML); thus, we further investigated the expression of TCR¦Æ-regulating factors, the distribution of the TCR¦Æ 3' untranslated region (3'-UTR) splice variants, and the expression level and correlation of the alternative splicing factor/splicing factor 2 (ASF/SF-2), Fc¦ÅRI¦Ã and ZAP-70 genes. TCR¦Æ 3'-UTR splice variants were identified in peripheral blood mononuclear cells (PBMCs) from 14 healthy individuals, 40 patients with CML and 22 patients with CML in complete remission (CML-CR) by RT-PCR. The expression level of the TCR¦Æ, Fc¦ÅRI¦Ã, ASF/SF-2 and ZAP-70 genes was analyzed by real-time quantitative PCR. While the expression of TCR¦Æ gene in the CML group was significantly lower than that in the healthy individual and CML-CR groups, a significantly higher expression of the FceRI¦Ã and ASF/SF-2 genes was found in the CML group. Two types of splicing forms were detected in all of the healthy individual CML-CR cases: wild type (WT) TCR¦Æ 3'-UTR and alternatively splieced (AS) TCR¦Æ 3'-UTR which have been alternatively splieced in the WT TCR¦Æ 3'-UTR . However, 35% of the CML cases contained only the wild type TCR¦Æ 3'-UTR isoform. Based on the TCR¦Æ 3'-UTR isoform expression characteristic, we divided the patients with CML into two subgroups: the WT+AS- CML group, containing patients that express only the wild type TCR¦Æ 3'-UTR, and the WT+AS+ CML group, which contained patients that expressed two TCR¦Æ 3'-UTR isoforms. A significantly different ASF/SF-2 and Fc¦ÅRI¦Ã gene expression pattern was found between the WT+AS- and WT+AS+CML groups. We concluded that defective TCR¦Æ expression may be characterized in the WT+AS-and WT+AS+CML subgroups by the different gene expression pattern. The overexpression of ASF/SF2, which alternatively splices the TCR¦Æ 3¡¯-UTR, is thought to participate in feedback regulation. The characteristics of TCR¦Æ 3'-UTR alternative splicing may be a novel immunological marker for the evaluation of the CML immune status.
%K ASF/SF-2gene
%K TCR¦Æ3¡ä-UTR
%K TCR¦Æ gene
%K Fc¦ÅRI¦Ã gene
%K Chronic myeloid leukemia
%K Real-time PCR
%U http://www.jhoonline.org/content/5/1/74