%0 Journal Article
%T Differential Expression of IL-6 and IGF-II in Guinea Fowl and Chicken
%A G. Goyal
%A V. Upmanyu
%A S.K. Singh
%A S.K. Shukla
%J International Journal of Poultry Science
%D 2010
%I 
%X Differential expression of IL-6 and IGF-II genes were studied in guinea fowl and broiler chicken using semi-quantitative analysis. A 219 bp fragment of IL-6 and 215 bp fragment of IGF-II were amplified in guinea fowl and broiler chicken using chicken specific primers. Semi-quantitative analysis revealed the adjusted Integral Density of 0.853 and 0.051 for IL-6 band in guinea fowl and broiler chicken respectively, revealing 16.62 fold higher IL-6 mRNA expression in LPS induced PBMCs from guinea fowl as compared to that from broiler. However, adjusted Integral Density of IGF-II band was 0.082 and 1.106 for IGF-II band in guinea fowl and broiler chicken respectively, which revealed 13.43 fold increase in IGF-II mRNA expression in LPS induced PBMCs in broiler chicken as compared to that in guinea fowl. Hence, guinea fowl showed higher expression of pro-inflammatory cytokine (IL-6) and lower expression of IGF-II in comparison to broiler chicken. These findings were as per expectation in view of much higher immuno-competence and lower growth rate in guinea fowl in comparison to chicken.
%K Differential expression
%K IL-6
%K IGF-II
%K guinea fowl
%U http://docsdrive.com/pdfs/ansinet/ijps/2010/756-760.pdf