%0 Journal Article
%T microRNA-214-mediated UBC9 expression in glioma
%A Zhiqiang Zhao
%A Xiaochao Tan
%A Ani Zhao
%A Liyuan Zhu
%A Bin Yin
%A Jiangang Yuan
%A Boqin Qiang & Xiaozhong Peng*
%J BMB Reports
%D 2012
%I Korean Society for Biochemistry and Molecular Biology
%X It has been reported that ubiquitin-conjugating enzyme 9 (Ubc9),the unique enzyme2 in the sumoylation pathway, is up-regulatedin many cancers. However, the expression and regulation ofUBC9 in glioma remains unknown. In this study, we found thatUbc9 was up-regulated in glioma tissues and cell lines comparedto a normal control. UBC9 knockdown by small interfering RNA(siRNA) affected cell proliferation and apoptosis in T98G cells.Further experiments revealed that microRNA (miR)-214 directlytargeted the 3' untranslated region (UTR) of UBC9 and that therewas an inverse relationship between the expression levels ofmiR-214 and UBC9 protein in glioma tissues and cells. miR-214overexpression suppressed the endogenous UBC9 protein andaffected T98G cell proliferation. These findings suggest thatmiR-214 reduction facilitates UBC9 expression and is involvedin the regulation of glioma cell proliferation
%K Cell proliferation
%K Glioma
%K miR-214
%K Sumoylation
%K UBC9
%U http://www.jbmb.or.kr/jbmb/pdf.php?data=MTMwMTIyMTZAcGRmX3JhaW50cmFjZV9sZWV5c0AlNUI0NS0xMSU1RDEyMTEyOTEzNTFfJTI4NjQxLTY0NiUyOUJNQl8xMi0wOTcucGRm