%0 Journal Article
%T Identification of interacting proteins of retinoid-related orphan nuclear receptor gamma in HepG2 cells
%A Ze-Min Huang1
%A #
%A Jun Wu2
%A #
%A Zheng-Cai Jia1
%A Yi Tian1
%A Jun Tang3
%A Yan Tang1
%A Ying Wang2
%A Yu-Zhang Wu1
%A * & Bing Ni1
%A *
%J BMB Reports
%D 2012
%I Korean Society for Biochemistry and Molecular Biology
%X The retinoid-related orphan nuclear receptor gamma (ROR¦Ã)plays critical roles in regulation of development, immunity andmetabolism. As transcription factor usually forms a proteincomplex to function, thus capturing and dissecting of theROR¦Ã protein complex will be helpful for exploring themechanisms underlying those functions. After construction ofthe recombinant tandem affinity purification (TAP) plasmid,pMSCVpuro ROR¦Ã-CTAP(SG), the nuclear localization ofROR¦Ã-CTAP(SG) fusion protein was verified. Followingisolation of ROR¦Ã protein complex by TAP strategy, sevencandidate interacting proteins were identified. Finally, the heatshock protein 90 (HSP90) and receptor-interacting protein 140(RIP140) were confirmed to interplay with ROR¦Ã byco-immunoprecipitation. Interference of HSP90 or/and RIP140genes resulted in dramatically decreased expression ofCYP2C8 gene, the ROR¦Ã target gene. Data from this studydemonstrate that HSP90 and RIP140 proteins interact withROR¦Ã protein in a complex format and function asco-activators in the ROR¦Ã-mediated regulatory processes ofHepG2 cells.
%K HSP90
%K Protein complex
%K RIP140
%K ROR¦Ã
%K TAP
%U http://www.jbmb.or.kr/jbmb/pdf.php?data=MTMwMTIyMTdAcGRmX3JhaW50cmFjZV9sZWV5c0AlNUI0NS02JTVEMTIwNjI2MDcwNV8lMjgzMzEtMzM2JTI5Qk1CXzExLTI0OS5wZGY=