%0 Journal Article
%T Parkin induces apoptotic cell death in TNF-¦Á-treated cervical cancer cells
%A Kyunghong Lee#
%A Min Ho Lee#
%A Yeo Wool Kang
%A Ki-Jong Rhee
%A Tae Ue Kim & Yoon Suk Kim*
%J BMB Reports
%D 2012
%I Korean Society for Biochemistry and Molecular Biology
%X Many malignant tumors become resistant to tumor necrosisfactor-alpha (TNF-¦Á)-induced cell death during carcinogenesis.In the present study, we examined whether parkin acts as atumor suppressor in HeLa cells, a human cervical cancer cellline resistant to TNF-¦Á-induced cell death. TNF-¦Á-treatmentalone did not affect HeLa cell viability. However, expressionof parkin restored TNF-¦Á-induced apoptosis in HeLa cells.Increased cell death was due to the activation of the apoptoticpathway. Expression of parkin in TNF-¦Á-treated HeLa cellsstimulated cleavage of the pro-apoptotic proteins caspase-8, -9,-3, -7 and poly ADP ribose polymerase (PARP). In addition,parkin expression resulted in decreased expression of thecaspase inhibitory protein, survivin. These results suggest thatparkin acts as a tumor suppressor in human cervical cancercells by modulating survivin expression and caspase activity.We propose that this pathway is a novel molecular mechanismby which parkin functions as a tumor suppressor.
%K Apoptosis
%K Caspase
%K Cervical cancer
%K Parkin
%K TNF-¦Á
%U http://www.jbmb.or.kr/jbmb/pdf.php?data=MTMwMTIyMTdAcGRmX3JhaW50cmFjZV9sZWV5c0AlNUI0NS05JTVEMTIwOTI3MTUzNV8lMjg1MjYtNTMxJTI5Qk1CXzEyLTEwNC5wZGY=