%0 Journal Article %T IFN¦Ã-mediated inhibition of cell proliferation through increased PKC¦Ä-induced overexpression of EC-SOD %A Yoon-Jae Jeon1 %A Hyun Yoo1 %A Byung Hak Kim1 %A Yun Sang Lee1 %A Byeongwook Jeon1 %A Sung-Sub Kim2 %A * & Tae-Yoon Kim1 %A * %J BMB Reports %D 2012 %I Korean Society for Biochemistry and Molecular Biology %X Extracellular superoxide dismutase (EC-SOD) overexpressionmodulates cellular responses such as tumor cell suppression andis induced by IFN¦Ã. Therefore, we examined the role of EC-SODin IFN¦Ã-mediated tumor cell suppression. We observed that thedominant-negative protein kinase C delta (PKC¦Ä) suppressesIFN¦Ã-induced EC-SOD expression in both keratinocytes andmelanoma cells. Our results also showed that PKC¦Ä-induced ECSODexpression was reduced by pretreatment with a PKCspecificinhibitor or a siRNA against PKC¦Ä. PKC¦Ä-induced ECSODexpression suppressed cell proliferations by the up-regulationof p21 and Rb, and the downregulation of cyclin A and D.Finally, we demonstrated that increased expression of EC-SODdrastically suppressed lung melanoma proliferation in an EC-SODtransgenic mouse via p21 expression. In summary, our findingssuggest that IFN¦Ã-induced EC-SOD expression occurs via activationof PKC¦Ä. Therefore, the upregulation of EC-SOD may beeffective for prevention of various cancers, including melanoma,via cell cycle arrest. %K EC-SOD %K IFN¦Ã %K Keratinocytes %K Melanoma %K PKC¦Ä %U http://www.jbmb.or.kr/jbmb/pdf.php?data=MTMwMTIyMTZAcGRmX3JhaW50cmFjZV9sZWV5c0AlNUI0NS0xMSU1RDEyMTEyOTEzNThfJTI4NjU5LTY2NCUyOUJNQl8xMi0wMDMucGRm