%0 Journal Article
%T Cross-immunizing potential of tumor MAGE-A epitopes recognized by HLA-A*02:01-restricted cytotoxic T lymphocytes
%A Ze-Min Huang1
%A #
%A Zheng-Cai Jia2
%A #
%A Jun Tang3
%A Yi Zhang1
%A Yi Tian1
%A Dong-Jing Ni1
%A Fang Wang2
%A Yu-Zhang Wu1
%A * & Bing Ni1
%A *
%J BMB Reports
%D 2012
%I Korean Society for Biochemistry and Molecular Biology
%X Almost all melanoma cells express at least one member of theMAGE-A antigen family, making the cytotoxic T cells (CTLs)epitopes with cross-immunizing potential in this family attractivecandidates for the broad spectrum of anti-melanomaimmunotherapy. In this study, four highly homologous peptides(P264: FLWGPRALA, P264I9: FLWGPRALI, P264V9:FLWGPRALV, and P264H8: FLWGPRAHA) from the MAGE-Aantigens were selected by homologous alignment. All fourpeptides showed high binding affinity and stability toHLA-A*02:01 molecules, and could prime CTL immune responsesin human PBMCs and in HLA-A*02:01/Kb transgenicmice. CTLs elicited by the four epitope peptides couldcross-lyse tumor cells expressing the mutual target antigens,except MAGE-A11 which was not tested. However, CTLs inducedby P264V9 and P264I9 showed the strongest target celllysis capabilities, suggesting both peptides may represent thecommon CTL epitopes shared by the eight MAGE-A antigens,which could induce more potent and broad-spectrum antitumorresponses in immunotherapy.
%K CTL
%K Epitope
%K HLA-A*02:01
%K MAGE-A antigens
%K Tumor
%U http://www.jbmb.or.kr/jbmb/pdf.php?data=MTMwMTIyMTdAcGRmX3JhaW50cmFjZV9sZWV5c0AlNUI0NS03JTVEMTIwNzI2MDgxOV8lMjg0MDgtNDEzJTI5Qk1CXzEyLTA2OC5wZGY=