%0 Journal Article
%T Alternative splicing and expression analysis of High expression of osmotically responsive genes1 (HOS1) in Arabidopsis
%A Jeong Hwan Lee
%A Soo Hyun Kim
%A Jae Joon Kim & Ji Hoon Ahn*
%J BMB Reports
%D 2012
%I Korean Society for Biochemistry and Molecular Biology
%X High expression of osmotically responsive genes1 (HOS1), akey regulator of low temperature response and flowering time,encodes an E3 ubiquitin ligase in Arabidopsis. Here, we reportcharacterization of a newly identified splice variant (HOS1-L)of HOS1. Comparative analyses revealed that HOS1-L has alonger 5' nucleotide sequence than that of the previouslyidentified HOS1 (HOS1-S) and that its protein sequence wasmore conserved than that of HOS1-S in plants. HOS1-Ltranscripts were spatio-temporally more abundant than thoseof HOS1-S. The recovery rate of HOS1-S expression was fasterthan that of HOS1-L after cold treatment. Diurnal oscillationpatterns of HOS1-L revealed that HOS1-L expression wasaffected by photoperiod. An in vitro pull-down assay revealedthat the HOS1-L protein interacted with the ICE1 protein.HOS1-L overexpression caused delayed flowering in wild-typeplants. Collectively, these results suggest regulation of HOS1expression at the post-transcriptional level.
%K AAB87130
%K Alternative splicing
%K BT005517
%K HOS1
%K Posttranscriptional regulation
%U http://www.jbmb.or.kr/jbmb/pdf.php?data=MTMwMTIyMTdAcGRmX3JhaW50cmFjZV9sZWV5c0AlNUI0NS05JTVEMTIwOTI3MTUzMF8lMjg1MTUtNTIwJTI5Qk1CXzEyLTA5Mi5wZGY=