%0 Journal Article
%T ISOLATION AND CLONING OF cDNA OF GENE ENCODING FOR METALLOTHIONEIN TYPE 2 FROM MELASTOMA AFFINE
%A SUHARSONO
%A NIKEN TRISNANINGRUM
%A LULUT DWI SULISTYANINGSIH
%A UTUT WIDYASTUT
%J BIOTROPIA : the Southeast Asian Journal of Tropical Biology
%D 2009
%I SEAMEO, BIOTROP
%X Metallothionein is an important protein for detoxifying heavy metal ions. h is research was conducted to isolate and clone cDNA of gene encoding for metallothionein type 2 from Melastoma affi ne . Total RNA was isolated from young leaves. Total cDNA was synthesized from the total RNA by reverse transcription. h e MaMt2 cDNA was successfully isolated by PCR technique. h e MaMt2 cDNA was inserted into pGEM-T Easy and the recombinant plasmid was successfully introduced into Escherichia coli DH5 ¦Á . DNA sequencing analysis showed that this cDNA is full length consisting of 246 pb encoding 81 amino acid residues. h is cDNA is identical to mRNA of AtMt2 from Arabidopsis thaliana. It does not contain any restriction sites found in the cloning sites of pGEM-T Easy. h e deduced protein of MaMT2 contains 14 cysteine residues distributed in the Cys-Cys, Cys-X-Cys, and Cys-X-X-Cys motifs
%K cDNA
%K metallothionein
%K Melastoma affine
%K cloning
%K cysteine
%U http://journal.biotrop.org/index.php/biotropia/article/view/64/43