%0 Journal Article
%T RP-HPLC METHOD DEVLOPMENT AND VALDATION OF BOSENTAN DRUGPRESENT IN TABLETS.
%A Karnaker reddy .T*
%J International Journal of Pharmacy and Technology
%D 2010
%I IJPT
%X A reverse phase isocratic high performance liquid chromatographic method was developed for the estimation of bosentan drug in tablet formulation. The separation was achieved by C-18 hypersil250*4.6mm, 5¦Ìm and methanol: potassium dihydrogen orthophosphate buffer pH 7.8 (60:40 v/v) as mobile phase, at a flow rate of 0.8 ml/min. Detection was carried out at 220 nm. Retention time of Bosentan was found to be 8.26 +or-1. The method has been validated for linearity, accuracy and precision. Linearity of bosenatan was in the range 50-150 mcg/ml. The mean recovery obtained for was 99.1. Developed method was found to be accurate, precise, selective and rapid for estimation of bosentan in tablets.
%K RP-HPLC
%K Bosentan
%K method development and validation INTRODUCTION: bosentan is competitive antagonist of endothelin-1
%U http://www.ijptonline.com/wp-content/uploads/2009/10/577-587.pdf