%0 Journal Article
%T DEVELOPMENT AND VALIDATION OF A HPLC METHOD FOR IN-VIVO STUDY OF DICLOFENAC POTASSIUM
%A Mohamed Aly Abd El Aziz Aly El Degwy*
%A Mohamed Aly Mahmoud Aly and Mamdouh Salah El Den
%J International Journal of Pharmaceutical Sciences and Research
%D 2013
%I Society of Pharmaceutical Sciences and Research
%X A High Performance Liquid Chromatographic method was developed for determination of Diclofenac Potassium in in-vivo study during bioavailability study. The chromatographic separation was conducted on Shimadzu Prominence LC 20 connected with PDA detector; using mixed column ODS/Cyano; ACE, (100 x 4.6 mm, 5 ¦Ìm). The mobile phase was isocratic consisted of Methanol: 50 mM potassium dihydrogen phosphate buffer, in ratio of (50 : 50 v/v) and was delivered to the system at a flow rate of 1.5 ml/min, with an injection volume of 20 ¦Ìl and the detection wavelength (¦Ë max) was 280 nm. Diazepam was used as internal standard. All assays were performed at ambient conditions. The calibration curve of Diclofenac Potassium in plasma was linear with correlation coefficient (r2) = 0.9996; over a concentration range of 0.25 - 3 ug/ml. The retention times for Diclofenac Potassium and the internal standard (Diazepam) were found to be 5.95 and 7.90 minutes, respectively. The mean recovery was found to be 90.57%.The relative standard deviation (RSD) was found to be < 2. The proposed method was validated and successfully applied to the determination of Diclofenac Potassium in human plasma for bioavailability studies.
%K Diclofenac Potassium
%K HPLC
%K Mixed column
%K Method Validation
%K In-vivo study
%U http://ijpsr.com/V4I2/12%20Vol.%204,%20Issue%202,%20Feb%202013,%20IJPSR,%20RA-2065,%20Paper%2012.pdf