%0 Journal Article
%T Multisubunit complex eEF1H in human glial tumors: from mRNA to protein
%A Veremieva M. V.
%A Malysheva T. A.
%A Zozulya Y. P.
%A Rozumenko V. D.
%J Biopolymers and Cell
%D 2010
%I Institute of Molecular Biology and Genetics, National Academy of Sciences of Ukraine
%X Aim. To investigate protein level of all subunits of the eukaryotic elongation translation factor eEF1H (eEF1A, eEF1B¦Á, eEF1B¦Âand eEF1B¦Ã) in glial tumors of human brain in comparison with normal brain. Methods. The eEF1H components content has been investigated in human glioblastoma clinical samples by Western blot analysis. Results. To determine the eEF1B¦Á, eEF1B¦Â and eEF1B¦Ã content, the polyclonal antibodies against all eEF1H subunits were obtained. The tendency of the eEF1B¦Ã protein level to increase in glioblastomas was observed. There were no significant differences in the eEF1A, eEF1B¦Á and eEF1B¦Â protein contents. Conclusions. In the previous report we analysed the expression of all eEF1H subunits in human glial brain tumor on the mRNA level. This study showed that eEF1B¦Ã was overexpressed while no significant changes in other eEF1H subunits were observed. It suggests a possible function of eEFBg which is cancer-related and is not connected with the functioning of eEF1H complex in translation.
%K translation elongation factor eEF1H
%K human glial brain tumors.
%U http://www.biopolymers.org.ua/archive/2010/04/ru/Veremieva.pdf