%0 Journal Article
%T Plasminogen/plasmin influence on platelet aggregation
%A Roka-Moya Y. M.
%A Zhernossekov D. D.
%A Grinenko T. V.
%J Biopolymers and Cell
%D 2012
%I Institute of Molecular Biology and Genetics, National Academy of Sciences of Ukraine
%X Aim. Determination of the influence of Glu- and Lys-plasminogen/ plasmin on the platelet aggregation induced by different agonists. Methods. Platelet aggregometry. Spectrophotometry. Results. We have shown the inhibitory effect of Lys-plasminogen on human platelet aggregation. The pro-enzyme action is related to the second wave of aggregation. The inhibitory effect of Lys-plasminogen possesses dosedependent manner in case of thrombin-induced aggregation. The inhibitory effect of pro-enzyme with ristocetin as an inductor has not been observed. Glu-plasminogen and plasmin do not influence on platelet aggregation under studied conditions. A serine protease inhibitor aprotinin does not change the inhibitory effect of Lys-plasminogen. Conclusions. The results proved the inhibitory effect of Lys-plasminogen on thrombin- and collagen-induced platelet aggregation. The absence of inhibitory effect of Lys-plasminogen in the case of ristocetin-induced aggregation leads us to conclusion, that GP Ib/IX is not involved into the inhibited aggregation pathway. The unchanged inhibitory effect of Lys-plasminogen in the presence of aprotinin excludes plasmin participation in the inhibitory phenomenon.
%K platelet aggregation
%K plasminogen
%K plasmin
%U http://www.biopolymers.org.ua/pdf/uk/28/5/352/