%0 Journal Article
%T Proteomics analysis of differentially expressed proteins in chicken trachea and kidney after infection with the highly virulent and attenuated coronavirus infectious bronchitis virus in vivo
%A Zhongzan Cao
%A Zongxi Han
%A Yuhao Shao
%A Xiaoli Liu
%A Junfeng Sun
%A Demin Yu
%A Xiangang Kong
%A Shengwang Liu
%J Proteome Science
%D 2012
%I BioMed Central
%R 10.1186/1477-5956-10-24
%X Fifty-eight differentially expressed proteins were identified. Results demonstrated that some proteins which had functions in cytoskeleton organization, anti-oxidative stress, and stress response, showed different change patterns in abundance from chicken infected with the highly virulent ck/CH/LDL/97I P5 strain and those given the embryo-passaged, attenuated P115 stain. In addition, the dynamic transcriptional alterations of 12 selected proteins were analyzed by the real-time RT-PCR, and western blot analysis confirmed the change in abundance of heat shock proteins (HSP) beta-1, annexin A2, and annexin A5.The proteomic alterations described here may suggest that these changes to protein expression correlate with IBV virus' virulence in chicken, hence provides valuable insights into the interactions of IBV with its host and may also assist with investigations of the pathogenesis of IBV and other coronavirus infections.Coronaviruses (CoVs) are enveloped single-stranded positive sense RNA viruses that belong to the family Coronaviridae in the order Nidovirales. They are able to infect humans as well as other animals, including cows, pigs, mice, and chickens, they generally cause respiratory infection, gastrointestinal, and neurological disorders of varying severity. Infectious bronchitis virus (IBV) was the first coronavirus to be discovered, and is classed among the Gamma coronaviruses on the basis of antigenic and genetic relatedness [1]. It is a major poultry pathogen and is probably endemic in all chicken-raising regions; it has a severe impact on poultry production, causing heavy economic losses. All strains of IBV are capable of infecting a large range of epithelial surfaces of chickens, such as those of the trachea, kidney, oviduct and proventriculus [2].Coronavirus infection has dramatic effects on host cell morphology, transcription and translation patterns, the cell cycle, cytoskeleton, suppression of interferon, and apoptosis pathways. Coronavirus infection
%K Infectious bronchitis virus
%K Proteomics
%K Chicken
%K Trachea
%K Kidney
%U http://www.proteomesci.com/content/10/1/24