%0 Journal Article
%T Protein kinase Cα downregulation via siRNA-PKCα released from foldable capsular vitreous body in cultured human retinal pigment epithelium cells
%A Chen X
%A Liu Y
%A Jiang Z
%A Zhou L
%J International Journal of Nanomedicine
%D 2011
%I Dove Medical Press
%X Xiaoqing Chen, Yaqin Liu, Zhaoxin Jiang, Lian Zhou, Jian Ge, Qianying GaoState Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-Sen University, Guangzhou, People¡¯s Republic of ChinaAbstract: We previously found that downregulation of protein kinase C¦Á (PKC¦Á) can inhibit retinal pigment epithelium (RPE) cell proliferation involved in the development of proliferative vitreoretinopathy (PVR). In this study, we tested whether PKC¦Á could be downregulated via small interfering RNA (siRNA)-PKC¦Á released from foldable capsular vitreous body (FCVB) in cultured human RPE cells. SiRNA-PKC¦Á content, determined by ultraviolet (UV) spectrophotometer, was released from FCVB containing 200, 300, 400, 500, and 600 nm siRNA-PKC¦Á in a time-dependent manner from 1 to 96 hours and a dose-dependent manner at five concentrations. The content (y) had a good linear relationship with time (x), especially in the 600 nm siRNA-PKC¦Á group (y = 16.214x, R2 = 0.9809). After treatment with siRNA-PKC¦Á released from FCVBs, the PKC¦Á was significantly decreased by RT-PCR, Western blot, and immunofluorescence analysis in RPE cells. These results indicate that PKC¦Á was significantly downregulated by siRNA-PKC¦Á released from FCVB in human RPE cells and provide us with a new avenue to prevent PVR.Keywords: small interfering RNA¨Cprotein kinase C¦Á, foldable capsular vitreous body, drug delivery system, retinal pigment epithelium, protein kinase C¦Á
%U http://www.dovepress.com/protein-kinase-calpha-downregulation-via-sirna-pkcalpha-released-from--a7709