%0 Journal Article
%T Mechanical isolation of capuchin monkey (Cebus apella) preantral ovarian follicles
%A Domingues S.F.S.
%A Ferreira H.S.
%A Muniz J.A.P.C.
%A Lima A.K.F.
%J Arquivo Brasileiro de Medicina Veterin¨¢ria e Zootecnia
%D 2003
%I Universidade Federal de Minas Gerais
%X The aim of this study was to adapt a mechanical procedure for the isolation of intact preantral follicles from Cebus apella ovaries. The interval effect of serial sections of the tissue chopper was tested on a number of preantral follicles isolated from ovaries (n=6) of three C. apella females, two prepubertal and one adult. Ovaries were divided into four equal parts and fragmented with a tissue chopper, adjusted for serial sections at intervals of 250, 500, 750 and 1,000¦Ìm, respectively. Isolated follicles were counted in a Neubauer's chamber and classified as primordial, primary or secondary. The number (mean¡ÀSE) of preantral follicles isolated from 1/4 ovary varied from 68,330+17,590 (at the 1,000¦Ìm cut interval) to 300,830+111,460 (at the 500¦Ìm cut interval. The mean diameter of the isolated preantral follicles varied from 11.6¦Ìm to 27.8¦Ìm.
%K monkey
%K preantral follicle
%K isolation
%K ovary
%K Cebus apella
%U http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352003000300009