%0 Journal Article
%T Long term stability of paraoxonase-1 and high-density lipoprotein in human serum
%A Piet K Beekhof
%A Maryana Gorshunska
%A Eug¨¨ne HJM Jansen
%J Lipids in Health and Disease
%D 2012
%I BioMed Central
%R 10.1186/1476-511x-11-53
%X In this study the stability of PON1 activity has been tested in human serum samples during storage up to 12£¿months at 3 commonly used temperatures, -20¡ãC, -70¡ãC and £¿196¡ãC. It was found that the stability of the PON1 activity is constant during 12£¿months of storage at £¿70¡ãC and £¿196¡ãC. Storage at £¿20¡ãC resulted in a small but statistically significant decrease after 6£¿months to about 94% of its original value. Nonetheless, the rank order between the samples at T£¿=£¿0 and 12£¿months remained the same. The same temperature dependence was found for the associated high-density lipoprotein.It can be concluded that £¿70¡ãC is the right temperature for storage to maintain the PON1 activity for at least one year. Storage at a lower temperature in liquid nitrogen (£¿196¡ãC) is not necessary.
%K Paraoxonase
%K High-density lipoprotein
%K Serum
%K Stability
%K Storage
%U http://www.lipidworld.com/content/11/1/53/abstract