%0 Journal Article
%T Danshensu inhibits acetaldehyde-induced proliferation and activation of hepatic stellate cell-T6
%A Li Zhang
%J Zhong Xi Yi Jie He Xue Bao
%D 2012
%I 
%X OBJECTIVE: To evaluate the effects of danshensu, the main component of the extract of Chinese medicine Salvia miltiorrhiza, on the proliferation and activation of hepatic stellate cells (HSCs).METHODS: The activation of HSC-T6 was induced by exposure to acetaldehyde. In the meantime, different doses of danshensu were added to the culture medium. After 24 h of treatment with danshensu in acetaldehyde, the viability of HSC-T6 cells was evaluated by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, the cell cycle was determined through flow cytometry, and the gene transcription levels of plasminogen activator inhibitor-1 (PAI-1), transforming growth factor-¦Â1 (TGF-¦Â1), urokinase-type plasminogen activator (uPA) and matrix metalloproteinase-2 (MMP-2) were analyzed by real-time quantitative polymerase chain reaction.RESULTS: The proliferation of HSCs induced by 200 ¦Ìmol/L acetaldehyde could be significantly inhibited by danshensu, and the percentage of HSCs in S phase was significantly increased as compared with the control cells (P£¼0.05), which were respectively evidenced by MTT assay and flow cytometry. Danshensu down-regulated the mRNA expression of TGF-¦Â1 and PAI-1 and up-regulated the uPA transcription level (P£¼0.01), while the transcription level of MMP-2 was not significantly affected in HSC-T6. CONCLUSION: Danshensu can inhibit the proliferation and activation of HSC-T6, as well as regulate some cytokines involved in extracellular matrix accumulation, which offers a potential therapeutic alternative for liver fibrosis.
%K danshensu
%K hepatocytes
%K cell proliferation
%K extracellular matrix
%K in vitro
%U http://www.jcimjournal.com/FullText2.aspx?articleID=jcim20121013