%0 Journal Article
%T EFFECT OF TWO PROTOCOLS OF CRYOPRESERVATION ON FERTILIZING CAPACITY OF STALLION (Equus caballus) SEMEN EFECTO DE DOS PROTOCOLOS DE CRIOPRESERVACI車N SOBRE LA CAPACIDAD FECUNDANTE DE SEMEN EQUINO (Equus caballus)
%A Giovanni Restrepo Betancur
%A Juan Esteban Duque Cort谷s
%A Juan David Montoya P芍ez
%J Revista Facultad Nacional de Agronom赤a, Medell赤n
%D 2012
%I Universidad Nacional de Colombia, Sede Medell赤n
%X . Semen cryopreservation is a fundamental process for the development of biotechnologies for assisted reproduction in horses. The use of cryopreservation techniques with changes in concentrations and the nature of the cryoprotectant, as well as, the different types of vials for storage of semen, have become an alternative to improve the protocols used. The objective of this work was to evaluate the effect of two protocols of cryopreservation (freezing and vitrification) on the fertilizing capacity of stallion semen. The study was conducted with horses of the Criollo Colombiano breed. For freezing was used a extender supplemented with egg yolk (4%) and dimethyl formamide (5%), and 0.5 mL straws as vials, whereas for vitrification, the extender was supplemented with egg yolk (8%) and dimethyl formamide (8%), and cryovials were used as carriers. As post thaw parameters were evaluated: progressive motility, vitality, normal morphology and integrity of the plasma membrane through the hypoosmotic swelling test (HOS). For statistical evaluation was fitted a generalized linear model (GLM) and means were compared by the Tukey test. Were found average percentages of progressive motility, vitality, normal morphology and HOS of 41.6 ㊣ 11.8 and 37 ㊣ 8.5, 54.3 ㊣ 10.2 and 52.3 ㊣ 7.8, 83.1 ㊣ 5.4 and 83.6 ㊣ 5.8, 41.7 ㊣ 9.8 and 38.9 ㊣ 3.6, for cryopreserved semen by freezing and vitrification, respectively. There were no statistically significant differences (P ≒ 0.05) between treatments for any of the parameters evaluated. The fertilizing capacity of equine semen cryopreserved by vitrification is comparable to that obtained by conventional freezing. Resumen. La criopreservaci車n de semen es un proceso fundamental en el desarrollo de biotecnolog赤as para la reproducci車n asistida en equinos. El uso de diferentes t谷cnicas de criopreservaci車n con cambios en las concentraciones y la naturaleza de los crioprotectores, as赤 como en los diferentes tipos de soportes para el almacenamiento del semen, se ha constituido en una alternativa para mejorar los protocolos empleados. El objetivo de este trabajo fue evaluar el efecto de dos protocolos de criopreservaci車n (congelaci車n y vitrificaci車n), sobre la capacidad fecundante del semen equino. El estudio se realiz車 con equinos de la raza Criollo Colombiano. Para la congelaci車n se emple車 un diluyente suplementado con de yema de huevo (4%) y dimetilformamida (5%), y pajillas de 0,5 mL como soportes; mientras que para la vitrificaci車n, el diluyente fue suplementado con yema de huevo (8%) y dimetilformamida (8%) y se usaron crioviale
%K Inseminaci車n artificial
%K calidad seminal
%K congelaci車n
%K vitrificaci車n
%K Artificial insemination
%K seminal quality
%K freezing
%K vitrification
%U http://www.scielo.org.co/scielo.php?script=sci_arttext&pid=S0304-28472012000200015