%0 Journal Article
%T Methylation of TIMP3 in esophageal squamous cell carcinoma
%A Eric Smith
%A Neville J De Young
%A Zi-Qiang Tian
%A Maria Caruso
%A Andrew R Ruszkiewicz
%A Jun-Feng Liu
%A Glyn G Jamieson
%A Paul A Drew
%J World Journal of Gastroenterology
%D 2008
%I Baishideng Publishing Group Co. Limited
%X AIM: To measure the frequency of DNA methylation of the tissue inhibitor of metalloproteinase 3 (TIMP3) promoter and relate this to any change of gene expression in esophageal squamous cell carcinoma in patients from a region of high incidence in China.METHODS: Cancer cell lines were treated with or without the demethylating reagent 5-aza-2กฏ-deoxycytidine. Methylation of the TIMP3 promoter was assessed in three regions by melt curve analysis and its expression was assessed by real-time RT-PCR. Tumors and proximal resection margins were obtained from 64 patients with esophageal squamous cell carcinoma from a region of high incidence in China. Methylation was assessed by melt curve analysis and expression by immunohistochemistry.RESULTS: Methylation in one of the three promoter regions assessed correlated with gene silencing in esophageal cell lines. A degree of methylation of TIMP3 was found in only four esophageal squamous cell carcinomas, and partial loss of TIMP3 protein expression in just one.CONCLUSION: Methylation and loss of expression of TIMP3 occurs infrequently in esophageal squamous cell carcinoma in a region of high incidence in China.
%K Esophageal squamous cell carcinoma
%K Immunohistochemistry
%K Methylation
%K Tissue inhibitor of metalloproteinase 3
%U http://www.wjgnet.com/1007-9327/full/v14/i2/203.htm