%0 Journal Article %T Simple Validated Method for Determination of Deoxynivalenol and Zearalenone in Some Cereals Using High Performance Liquid Chromatography %A Ahmed S. Sebaei %A Ahmed M. Gomaa %A Gehad G. Mohamed %A F.A. Nour El-Dien %J American Journal of Food Technology %D 2012 %I Science publications %X Deoxynivalenol (DON) and zearalenone (ZON) are two of the five most important naturally occurring mycotoxins in human foods and animal feeds produced by Fusarium species. The aim of the present study was to develop a fast extraction and a cheap method that can be applied for the determination of DON and ZON in cereals using High Performance Liquid Chromatography (HPLC) using diode array and fluorescence detectors. The method validation performance was tested on three varieties of cereal samples (maize, rice and wheat). The within-laboratory reproducibility expressed as relative standard deviation (RSDWR) was less than 10% for both mycotoxins (9% for DON and 7% for ZON). The method is linear from the Limit of Quantification (LOQ) from 200 to 2000 ¦Ìg kg-1 levels for DON and 20 to 400 ¦Ìg kg-1 levels for ZON. The Limits of Detections (LOD) for maize, rice and wheat samples were 24, 32 and 40 ¦Ìg kg-1 for DON, respectively and 3 ¦Ìg kg-1 for ZON. The measurement of uncertainty in terms of expanded uncertainty expressed as relative standard deviation (at 95% confidence level and coverage factor of k = 2) is in the range of ¡À31% for DON and ¡À25% for ZON, the method is economically useful in ZON and DON determination in safety and quality assurance programs. %K Deoxynivalenol %K method validation %K mycotoxins %K zearalenone %K cereals %U http://docsdrive.com/pdfs/academicjournals/ajft/2012/668-678.pdf