%0 Journal Article
%T A neurochemical map of the developing amphioxus nervous system
%A Simona Candiani
%A Luca Moronti
%A Paola Ramoino
%A Michael Schubert
%A Mario Pestarino
%J BMC Neuroscience
%D 2012
%I BioMed Central
%R 10.1186/1471-2202-13-59
%X By single and double in situ hybridization experiments, we identified glutamatergic, GABAergic/glycinergic, serotonergic and cholinergic neurons in developing amphioxus. In addition to characterizing the distribution of excitatory and inhibitory neurons in the developing amphioxus CNS, we observed that cholinergic and GABAergic/glycinergic neurons are segmentally arranged in the hindbrain, whereas serotonergic, glutamatergic and dopaminergic neurons are restricted to specific regions of the cerebral vesicle and the hindbrain. We were further able to identify discrete groups of GABAergic and glutamatergic interneurons and cholinergic motoneurons at the level of the primary motor center (PMC), the major integrative center of sensory and motor stimuli of the amphioxus nerve cord.In this study, we assessed neuronal differentiation in the developing amphioxus nervous system and compiled the first neurochemical map of the amphioxus CNS. This map is a first step towards a full characterization of the neurotransmitter signature of previously described nerve cell types in the amphioxus CNS, such as motoneurons and interneurons.
%K Branchiostoma
%K Cephalochordate
%K Chordate evolution
%K Lancelet
%K Neural patterning
%K Neurotransmitter
%U http://www.biomedcentral.com/1471-2202/13/59/abstract