%0 Journal Article
%T Muta  o BRAF em pacientes idosos submetidos ¨¤ tireoidectomia BRAF mutation in the elderly submitted to thyroidectomy
%A Antonio Augusto T. Bertelli
%A Antonio Jos¨¦ Gon£¿alves
%A Marcelo B. Menezes
%A Murilo R. Melo
%J Revista do Col¨¦gio Brasileiro de Cirurgi£¿es
%D 2013
%I Col¨¦gio Brasileiro de Cirurgi?es
%X OBJETIVO: Avaliar a frequ¨ºncia da muta  o V600E do gene BRAF em pacientes com mais de 65 anos de idade submetidos ¨¤ tireoidectomia, correlacionando sua presen a ou aus¨ºncia com as diferentes les es histol¨®gicas, com as variantes e com fatores progn¨®sticos do carcinoma papil¨ªfero. M¨¦TODOS: Foram avaliados 85 pacientes com mais de 65 anos de idade submetidos ¨¤ tireoidectomia, analisando a muta  o BRAF V600E atrav¨¦s de rea  o de PCR-RT realizada ap¨®s a extra  o do DNA dos blocos de parafina. RESULTADOS: Detectou-se aus¨ºncia ou presen a da muta  o BRAF V600E em 47 pacientes (55,3%). Entre os 17 carcinomas papil¨ªferos estudados, sete apresentavam a muta  o (41,2%). Demonstrou-se associa  o estat¨ªstica entre a presen a desta muta  o e a variante cl¨¢ssica do carcinoma papil¨ªfero, al¨¦m de tend¨ºncia de associa  o com o extravasamento tireoideano. CONCLUS O: A muta  o BRAF nos pacientes idosos tamb¨¦m ¨¦ exclusiva do carcinoma papil¨ªfero e tem frequ¨ºncia expressiva. Al¨¦m disso, est¨¢ relacionada ¨¤ variante cl¨¢ssica e, possivelmente, ao extravasamento tireoideano. OBJECTIVE: To evaluate the frequency of the BRAF V600E mutation in patients over 65 years of age undergoing thyroidectomy, correlating its presence or absence with the different histologic lesions, their variants and with prognostic factors of papillary carcinoma. METHODS: We evaluated 85 patients over 65 years of age who underwent thyroidectomy, analyzing the BRAF V600E mutation by RT-PCR performed after DNA extraction from the paraffin blocks. RESULTS: The study detected the presence or absence of BRAF V600E mutation in 47 patients (55.3%). Among the 17 papillary carcinomas studied, seven had the mutation (41.2%). There was a statistical association between the presence of this mutation and the classic variant of papillary carcinoma, and a trend of association with thyroid extravasation. CONCLUSION: BRAF mutation in the elderly is also exclusive of papillary carcinoma and is often significant. Furthermore, it is related to the classic variant and possibly to thyroid extravasation.
%K Glandula tireoide
%K Neoplasias da glandula tireoide
%K Tireoidectomia
%K Prote¨ªnas proto-oncog¨ºnicas B-raf
%K Muta  o
%K Thyroid gland
%K Thyroidectomy
%K Neoplasms of the thyroid gland
%K Proteins Proto-oncogene B-raf
%K Mutation
%K Elderly
%U http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-69912013000200005