%0 Journal Article
%T Effects of ambroxol on biofilm adhesion and viability of Pseudomonas aeruginosa quorum sensing defective strain
%A Qi LU
%A Hai-ying ZHONG
%A Li-hua LIN
%A Ya-yin LIN
%J Medical Journal of Chinese People's Liberation Army
%D 2013
%I Editorial Board of Medical Journal of Chinese People's Liberation Army
%X ObjectiveĦĦTo investigate the effects of ambroxol on the biofilm viability and pristine adhesion of Pseudomonas aeruginosa wild (PAO1) and quorum sensing defective strain (QS, gene deletion of  lasI and  rhlI). MethodsĦĦThe biofilm was treated by different concentrations (0, 1.875, 3.75mg/ml) of ambroxol. The number of colony was measured with agar plate, multifunction fluorometer was used to measure the fluorescence intensity of PAO1 and QS strains at the bottom of 96-well plate. The adhesion ratio (%) was calculated to determine the effects of ambroxol on bacterial biofilm adhesion. ResultsĦĦAmbroxol treatment reduced the survival rate of the mutant strains compared to that of wild strain, even though the QS strain had increased the adhesion in the presence of ambroxol compared to that of wild strain (P<0.05). ConclusionĦĦAmbroxol has a property of significantly antagonizing quorum-sensing system, suggesting that it might be of importance in treatment against chronic Pseudomonasaeruginosainfections.
%K ambroxol
%K Pseudomonas aeruginosa
%K bacterial adhesion
%K quorum sensing
%U http://www.plamj.org/index.php/plamj/article/view/740