%0 Journal Article
%T Effects of liposome-adriamycin (L-ADM) and thermotherapy on glioma cells: an experimental study
%A Zheng-hua SHI
%A Jian-ning ZHANG
%J Medical Journal of Chinese People's Liberation Army
%D 2013
%I Editorial Board of Medical Journal of Chinese People's Liberation Army
%X Objective ””To observe the effects of liposome-adriamycin (L-ADM) and thermotherapy on proliferation and apoptosis of SWO-38 glioma cells. Methods ””The SWO-38 glioma cells were cultivated in vitro. The effects of thermotherapy (43”ę), ADM chemotherapy, L-ADM chemotherapy, thermotherapy + ADM chemotherapy, and thermotherapy + L-ADM chemotherapy on the cell proliferation and apoptosis were observed. The working concentration of ADM and L-ADM, and the cell proliferation rate were determined by MTT method. The apoptotic rate was determined by flow cytometry. Results ””The proliferation inhibition rate of thermotherapy + L-ADM chemotherapy and thermotherapy + ADM chemotherapy was 80.16%”Ą3.78% and 62.09%”Ą3.05%, respectively, and it was significantly higher than that of L-ADM chemotherapy (40.27%”Ą2.32%), ADM chemotherapy (30.56%”Ą2.03%) or thermotherapy (16.51%”Ą1.26%, P<0.05), and the proliferation inhibition rate of thermotherapy + L-ADM chemotherapy was higher than that of thermotherapy + ADM chemotherapy (P<0.05). The apoptotic rate of thermotherapy + L-ADM chemotherapy and thermotherapy + ADM chemotherapy was 84.19%”Ą2.69% and 60.29%”Ą1.47%, respectively, and it was significantly higher than that of L-ADM chemotherapy (46.72%”Ą2.09%), ADM chemotherapy (35.09%”Ą1.46%) and thermotherapy (17.85%”Ą0.78%, P<0.05), and the apoptotic rate of thermotherapy + L-ADM chemotherapy was higher than that of thermotherapy + ADM chemotherapy (P<0.05). Conclusion ””Thermotherapy can enhance proliferation inhibition and apoptosis induction effect of ADM and L-ADM on SWO-38 glioma cells, and this effect is even stronger with L-ADM.
%K doxorubicin
%K thermochemotherapy
%K cell proliferation
%K apoptosis
%U http://www.plamj.org/index.php/plamj/article/view/663