%0 Journal Article
%T Expression of Cripto-1 in esophageal carcinoma and its relationship with epithelial-mesenchymal transformation
%A Wang-sheng CHEN
%A Zhong-xue FU
%A Chun HUANG
%A Kun-ming WEN
%J Medical Journal of Chinese People's Liberation Army
%D 2013
%I Editorial Board of Medical Journal of Chinese People's Liberation Army
%X Objective ¡¡To investigate the expression of Cripto-1 in esophageal carcinoma and its relationship with epithelial-mesenchymal transformation (EMT). Methods ¡¡RT-PCR and Western blotting were used to examine the expression levels of Cripto-1 in carcinoma, paraneoplastic and normal esophageal mucosa tissue of 41 patients with esophageal carcinoma. The protein expressions of Cripto-1 and EMT markers such as N-cadherin, Vimentin and E-cadherin were detected by immunohistochemistry, and the clinical pathologic parameters were simultaneously analyzed. The correlation between Cripto-1 and the expression of N-cadherin, Vimentin and E-cadherin were also analyzed. Results ¡¡The expression levels of Cripto-1 mRNA and protein were significantly higher in esophageal carcinoma (0.35¡À0.08 and 0.62¡À0.06) than in paraneoplastic (0.22¡À0.04 and 0.45¡À0.07) and normal esophageal mucosa tissue (0.13¡À0.03 and 0.33¡À0.05, P<0.05). Immunohistochemistry revealed that the positive expression rates of Cripto-1, N-cadherin, Vimentin and E-cadherin protein in esophageal carcinoma were 53.7%, 51.2%, 61.0% and 36.6% respectively. The expression of Cripto-1 showed a positive correlation with N-cadherin (r=0.463, P<0.05) and Vimentin (r=0.460, P<0.05), while a negative correlation with E-cadherin (r=£­0.310, P<0.05) was found. The expression of Cripto-1 also showed a significant correlation with lymph node metastasis, distant metastasis and clinical stage (P<0.05). Conclusion ¡¡The expression of Cripto-1 is closely related to the carcinogenesis and development of esophageal carcinoma, and may promote the invasion and metastasis of esophageal carcinoma by regulation of EMT.
%K esophageal neoplasms
%K stromal cells
%K epithelial cells
%U http://www.plamj.org/index.php/plamj/article/view/683