%0 Journal Article
%T A quantitative view of the transcriptome of Schistosoma mansoni adult-worms using SAGE
%A Elida PB Ojopi
%A Paulo SL Oliveira
%A Diana N Nunes
%A Apu£¿ Paquola
%A Ricardo DeMarco
%A Sheila P Greg¨®rio
%A Karina A Aires
%A Carlos FM Menck
%A Luciana CC Leite
%A Sergio Verjovski-Almeida
%A Emmanuel Dias-Neto
%J BMC Genomics
%D 2007
%I BioMed Central
%R 10.1186/1471-2164-8-186
%X After extracting mRNA from pooled male and female adult-worms, a SAGE library was constructed and sequenced, generating 68,238 tags that covered more than 6,000 genes expressed in this developmental stage. An analysis of the ordered tag-list shows the genes of F10 eggshell protein, pol-polyprotein, HSP86, 14-3-3 and a transcript yet to be identified to be the five top most abundant genes in pooled adult worms. Whereas only 8% of the 100 most abundant tags found in adult worms of S. mansoni could not be assigned to transcripts of this parasite, 46.9% of the total ditags could not be mapped, demonstrating that the 3 sequence of most of the rarest transcripts are still to be identified. Mapping of our SAGE tags to S. mansoni genes suggested the occurrence of alternative-polyadenylation in at least 13 gene transcripts. Most of these events seem to shorten the 3 UTR of the mRNAs, which may have consequences over their stability and regulation.SAGE revealed the frequency of expression of the majority of the S. mansoni genes. Transcriptome data suggests that alternative polyadenylation is likely to be used in the control of mRNA stability in this organism. When transcriptome was compared with the proteomic data available, we observed a correlation of about 50%, suggesting that both transcriptional and post-transcriptional regulation are important for determining protein abundance in S. mansoni. The generation of SAGE tags from other life-cycle stages should contribute to reveal the dynamics of gene expression in this important parasite.Quantitative and qualitative transcriptome analyses reveal some of the most important biological aspects of an organism. Transcriptome examination is crucial for the understanding of significant biological processes, allowing the study of transcription/translation relationships, the dynamics of gene expression and, an important feature in parasites, a quantitative evaluation of the expression of genes that are potential targets for drugs or
%U http://www.biomedcentral.com/1471-2164/8/186