%0 Journal Article
%T Directed migration of human neural progenitor cells to interleukin-1¦Â is promoted by chemokines stromal cell-derived factor-1 and monocyte chemotactic factor-1 in mouse brains
%A Wu Yumei
%A Chen Qiang
%A Peng Hui
%A Dou Huanyu
%J Translational Neurodegeneration
%D 2012
%I BioMed Central
%R 10.1186/2047-9158-1-15
%X Background Neurogenesis, including the proliferation, migration and differentiation of neural progenitor cells (NPCs), is impaired in HIV-1 associated dementia (HAD). We previously demonstrated HIV-1-infected macrophages (HIV-MDM) regulate stromal cell-derived factor 1 (SDF-1) production in astrocytes through Interleukin-1¦Â (IL-1¦Â). Chemokines are known to induce NPC migration; however, it remains unclear how chemokines produced in inflammation regulate NPC migration. Methods The secretion of SDF-1 and Monocyte chemotactic preotein-1 (MCP-1) in astrocytes upon IL-1¦Â stimulation was measured by ELISA assay. Human NPCs were injected parallel along with IL-1¦Â, SDF-1 or MCP-1 intracranially into basal ganglion 1 mm apart in SCID mice, and immunofluorescent staining was used to study the survival and migration of injected human NPCs. Results SDF-1 and MCP-1 are secreted by astrocytes upon IL-1¦Â stimulation in a time-dependent manner. Injected human NPCs survived in SCID mice and migrated towards sites of IL-1¦Â, SDF-1 and MCP-1 injection. Conclusions In conclusion, chemokines SDF-1 or MCP-1 secreted by astrocytes in the presence of IL-1¦Â injection are attractive to NPCs injected into SCID mouse brains, suggesting that SDF-1 and MCP-1 play important roles in NPC migration during neuroinflammation.
%U http://www.translationalneurodegeneration.com/content/1/1/15