%0 Journal Article %T Identification of chemosensory receptor genes in Manduca sexta and knockdown by RNA interference %A Natalie Howlett %A Katherine L Dauber %A Aditi Shukla %A Brian Morton %A John I Glendinning %A Elyssa Brent %A Caroline Gleason %A Fahmida Islam %A Denisse Izquierdo %A Sweta Sanghavi %A Anika Afroz %A Aanam Aslam %A Marissa Barbaro %A Rebekah Blutstein %A Margarita Borovka %A Brianna Desire %A Ayala Elikhis %A Qing Fan %A Katherine Hoffman %A Amy Huang %A Dominique Keefe %A Sarah Lopatin %A Samara Miller %A Priyata Patel %A Danielle Rizzini %A Alyssa Robinson %A Karimah Rokins %A Aneta Turlik %A Jennifer H Mansfield %J BMC Genomics %D 2012 %I BioMed Central %R 10.1186/1471-2164-13-211 %X Here, we sequenced transcriptomes from adult and larval chemosensory tissues and identified chemosensory genes based on sequence homology. We also used dsRNA feeding as a method to induce RNA interference in larval chemosensory tissues.We report identification of new chemosensory receptor genes including 17 novel odorant receptors and one novel gustatory receptor. Further, we demonstrate that systemic RNA interference can be used in larval olfactory neurons to reduce expression of chemosensory receptor transcripts. Together, our results further the development of M. sexta as a model for functional analysis of insect chemosensation. %U http://www.biomedcentral.com/1471-2164/13/211/abstract