%0 Journal Article
%T Desarrollo de un modelo de aterosclerosis inducida por inmunocomplejos de forma pasiva en ratones BALB/CBJ transg谷nicos al ant赤geno de superficie de la hepatitis B
%A Liudmila L車pez Matilla
%A Lien L車pez Matilla
%A Antonio Gonz芍lez Griego
%A V赤ctor Manuel Sosa
%J Revista Cubana de Investigaciones Biomˋˋdicas
%D 2002
%I ECIMED
%X Se obtuvieron anticuerpos-HBs a partir del desarrollo de ascitis en ratones BALB/CBJ. Se emplearon 2 grupos de animales BALB/CBJ transg谷nicos al AgsHb, de 8 meses de edad y un r谷gimen de agua y comida ad libitum. A un grupo se le inocul車 Ac anti-HBs con una dosis doble a la de su concentraci車n de AgsHb y al otro grupo se le inocul車 soluci車n salina. Se realizaron 7 inoculaciones con un intervalo de 5 d entre ellas por la vena caudal. Una semana despu谷s de la 迆ltima inoculaci車n se realiz車 extracci車n de sangre y se les determin車 inmunocomplejos circulantes en plasma. Se les practic車 eutanasia y se les extrajo las aortas donde se comprob車 la formaci車n de placas de ateromas de forma macrosc車pica por observaci車n al estereoscopio y microsc車picamente por tinci車n con hematosilina-eosina. A partir de estos resultados se concluy車 que se logr車 un modelo de aterosclerosis en ratones BALB/CBJ Tg al AgsHb por formaci車n de inmunocomplejos de forma pasiva. Anti-HBs antibody was obtained starting from the development of ascites in BALB/CBJ mice. Two groups of 8-month-old BALB/CBJ animals transgenic to HBsAg on an ad libitum water and food regimen were used. A group was inoculated anti-HBs antibody with a dose that doubled its concentration of HBsAg, whereas the other group was inoculated saline solution. 7 innoculations were made by the caudal vein with an interval of 5 days between them. A week after the last inoculation, blood was extracted and the immunocomplexes circulating in plasma were determined. Euthanasia was practiced and the aortas were extracted. The formation of plaques of atheromas was proved in a macroscopic way by observation on the stereoscopico and, microscopically, by staining with haematoxylin-eosin. Based on these results, it was concluded that an atherosclerosis model was attained in BALB/CBJ mice transgenic to HBsAg by the formation of immunocomplexes in a passive way.
%K ATEROSCLEROSIS
%K TECNICAS INMUNOLOGICAS
%K ANTIGENOS DE SUPERFICIE DE LA HEPATITIS B
%K RATONES TRANSGENICOS
%K ATHEROSCLEROSIS
%K IMMUNOLOGIC TECHNIQUES
%K HEPATITIS B SURFACE ANTIGENS
%K MICE
%K TRANSGENIC
%U http://scielo.sld.cu/scielo.php?script=sci_arttext&pid=S0864-03002002000100007