%0 Journal Article
%T Estudio molecular del gen MLL en 30 pacientes con leucemias agudas Molecular study of MLL gen in 30 patients with acute leukemias
%A Raquel Lev車n Herrera
%A Alejandro Gonz芍lez Otero
%A Edgardo Espinosa Mart赤nez
%A Porfirio Hern芍ndez Ram赤rez
%J Revista Cubana de Hematologˋ-a, Inmunologˋ-a y Hemoterapia
%D 2000
%I Editorial Ciencias M谷dicas
%X Los reordenamientos del gen MLL en la banda cromos車mica 11q23 son frecuentes en leucemias agudas (LA) en ni os y en las LA secundarias desarrolladas despu谷s de la terapia con inhibidores de la enzima topoisomerasa II. En menor medida tambi谷n se aprecia en adultos con LA. La presencia de estos reordenamientos se considera un indicador de mal pron車stico asociado con resultados cl赤nicos desfavorables, por ello es muy importante realizar su determinaci車n en las LA. En este trabajo mostramos los resultados preliminares de la introducci車n del estudio del gen MLL en nuestro pa赤s mediante la t谷cnica de Southern. Analizamos ADN de 30 pacientes con LA, incluidos ni os y adultos, que en el momento del estudio se encontraban al debut o en reca赤da. El estudio molecular se realiz車 con la sonda FA4, que es un inserto gen車mico del gen MLL. S車lo uno de los 30 pacientes mostr車 bandas de reordenamiento con 2 enzimas de restricci車n diferentes, el resto mostr車 el gen MLL en configuraci車n germinal. Es interesante destacar que el paciente con el reordenamiento era un ni o con leucemia mielobl芍stica aguda subtipo M5b, lo cual concuerda con la literatura, donde se describe que estos reordenamientos est芍n estrechamente correlacionados con los subtipos mielomonoc赤tico (M4) y monoc赤tico (M5) de leucemia mieloide aguda (LMA) Rearrangements of MLL gen in llq23 chromosomal band are frequents in childhood type of acute leukemia (AL) and in secondary AL, developed after therapy with II topoisomerase enzyme. To a lesser extent also is seen in adults with AL. Presence of theses rearrangements is considered to be a worse prognosis indicator, associated with unfavourable clinical results, that is why it is very important to carry our its assessment in AL. In this paper authors present preliminary results from introduction of study on MLL gen in our country through Southern technique. DNA from 30 patients was analized, including children and adults, that at the very moment when study was performed, they were in the onset or in relapse. Molecular study was carried out using FA4 probe, which is a genomic insertion of MLL gen. Only one out of 30 patients, presented rearrangement band with 2 distinct restriction enzymes, remainder showed MLL gen in germinal form. It is interesting to underline that patient with rearrangement was a child presenting subtype M5b acute myeloblastic leukemia, which agree with literature showing that these rearrangements are closely correlated to subtype myelomonocytic (M4) and monocytic (M5) of acute myeloid leukemia (AML)
%K REORDENAMIENTO GENICO
%K ENFERMEDADES HEMATOLOGICAS
%K LEUCEMIA LINFOCITICA
%K GENE REARRANGEMENT
%K HEMATOLOGIC DISEASES
%K LEUKEMIA
%K LYMPHOCYTIC
%U http://scielo.sld.cu/scielo.php?script=sci_arttext&pid=S0864-02892000000100004