%0 Journal Article
%T Influence of Uracil in Fermentation Media on ¦Â-Glucan Production by Agrobacterium Radiobacter A 1.5 and Agrobacterium sp. Bro 1.2.1
%A Kusmiati
%A Salmah Muhamad
%A Sukma Nuswantara
%A Swasono R.Tamat
%J Makara Seri Sains
%D 2007
%I Universitas Indonesia
%X Optimum ¦Â-glucan production can be achieved by an optimum condition in the fermentation media. Uracil, as a precursor of UDP-glucose, may act as a glucose donor in the formation of polysaccharides such as ¦Â-glucan. It is expected that addition of certain quantity of uracil into the fermentation media in a suitable growth phase of Agrobacterium radiobacter A 1.5 and Agrobacterium sp. Bro 1.2.1, will significantly increase the ¦Â-glucan production. In this investigation, 0.025%; 0.05% or 0.1% of uracil were added into the fermentation media during the logarithmic phase (24 hour) or stationary phase (46 hour) of growth. The ¦Â-glucan product was evaluated from the ¦Â-glucan (crude) dry-weight and from the ¦Â-glucan content. Beta-glucan content was determined as glucose by the Hisamatsu-AOAC and HPLC methods. The highest ¦Â-glucan (crude) dry-weight produced by the A. 1.5 was in a medium containg 0.025% uracil (24 hour), whilst by the A. Bro 1.2.1 was in a medium containg 0.1% uracil (46 hour), both higher than control. The highest ¦Â-glucan content produced by the A. 1.5 (27.03%) was in a medium containg 0.025% uracil (46 hour), while control produced only 23.28%. The highest ¦Â-glucan content produced by the Bro 1.2.1 (29.34%) was in a medium containg 0.025% uracil (24 hour), while control produced only 28.75%. Two-way anova analysis showed that there were no significant influence difference (¦Á = 0,05) from various concentration of uracil in either growth phases, to the yield of ¦Â-glucan (crude) dry-weight nor to the ¦Â-glucan equivalent glucose content.
%K Agrobacterium radiobacter A 1.5
%K Agrobacterium sp. Bro 1.2.1 mutant
%K ¦Â-glucan
%K Uracil
%U http://journal.ui.ac.id/science/article/viewFile/268/264